铅对雄性大鼠睾丸重量影响的多水平Meta回归分析.DOC

PAGE PAGE 1 - 铅对雄性大鼠睾丸重量影响的Meta回归分析 焦建栋 江苏省无锡市疾病预防控制中心，无锡，241023 摘要： [目的] 综合分析铅对雄性大鼠睾丸重量的影响。[方法] 应用meta回归方法对12个研究方法相同有关铅对雄性生殖功能影响的病例对照研究进行综合分析。计算控制年龄、体重的影响后的合并效应值，并进行异质性检验及偏倚分析。[结果] 单纯随机效应模型显示铅暴露后雄性大鼠睾丸重量平均降低量(d)为0.047g(95%CI:0.0044－0.0890，P0.05)，未成年组大鼠暴露后睾丸重量降低0.06g，大于性成熟组的0.025g(P＝0.027)，随着体重增加，大鼠睾丸重量受铅暴露影响越来越小(b=-0.0004,P=0.024)。与单纯随机效应模型相比，控制了年龄、体重的影响后meta分析研究间变异逐渐减少。 [结论] 运 关键词: 铅；雄性生殖功能；大鼠；睾丸重量；meta回归 EFFECT OF LEAD EXPOSURE ON TESTES WEIGHT IN MALE RATS: A META-REGRESSION ANALYSIS JIAO Jian-dong（Wuxi Center for Disease Control and Prevention, Wuxi, 214023） Abstract: [Objective] To comprehensively analyze the effect of lead exposure on testes weight of rats. [Methods] 12 case-control studies concerning male reproduction, with same experimental method, were selected to perform meta-regression analysis. A pooled effect was evaluated after controlling age and body weight. Heterogeneity test and bias analysis were also untaken. [Results] Random effect model showed the mean difference of testes weight was 0.047g (P0.05), with 95%CI was 0.0044 to 0.0890. The mean difference of testes weight was 0.06g in non-matured group, whereas the value was 0.025 in matured group (P=0.027). The effect size was much lower along with the body weight, showed by meta-regression model, the coefficient was -0.0004. The estimate value of between-study variance was significantly reduced after controlling age and body weight. [Conclusion] Age and body weight were defined as two important covariates in meta-regression model, after controlling their effect, lead was recognized to make rats testes weight lower, so as to damage male reproduction. Key Words: Lead; Male reproduction; Rat; Testes weight; Meta regression 作为一种内分泌干扰物(endocrine disrupters，EDs)，铅具有雄(男)性生殖毒性，可以影响雄性生殖结局。铅的生殖毒作用机制尚不十分清楚，但目前认为与铅对生殖器官的直接毒作用和对生殖内分泌的影响有关?铅可以通过血睾丸屏障，直接蓄积于睾丸的任何部位，干扰精子的生成和发育过程[1]。很多学者对铅暴露对睾丸重量的影响进行了研究，但由于各单独研究的对象、方法有所差异，不同研究之间所得结论却不尽相同[2][3]。本研究通过收集1980以来相关文献数据，采用Meta回归分析方法，来明确铅暴露与雄性大鼠睾丸重量的关系及其影响因素。 1 材