甜菜夜蛾地理种群基因流和种群扩张历史分析.docVIP

甜菜夜蛾地理种群基因流和种群扩张历史分析 　　摘要：利用mtDNA COI基因序列对采自我国7个地理种群的甜菜夜蛾（Spodoptera exigua Hübner）遗传多样性与种群扩张历史进行研究。结果表明，比对206条序列（序列长度为547 bp），共发现8个变异位点，定义5个单倍型，其中包括3个共享单倍型和2个独有单倍型；群体遗传多样性较低（Hd=0.320，π=0.000 70，Kxy=0.383）。群体间存在一定的遗传分化（FST=0.398）、基因流水平较低（Nm=0.378）。AMOVA分析表明，甜菜夜蛾遗传变异主要来自种群内，种群间变异水平较低。中性检验（Tajima’s D=-1.576，P0.05；Fu and Li′s F=-3.834，P0.05）与错配分布分析表明，我国北方甜菜夜蛾种群曾经历种群近期扩张。 　　关键词：甜菜夜蛾（Spodoptera exigua Hübner）；COI基因；遗传结构；遗传分化 　　中图分类号：S433.4 文献标识码：A 文章编号：0439-8114（2015）14-3460-06 　　DOI：10.14088/j.cnki.issn0439-8114.2015.14.032 　　Gene Flow and Population Expansion History of the Beet Armyworm， 　　Spodoptera exigua Hübner 　　WANG Xing-yaa，ZHOU Li-hongb 　　（a. Institute of Plant Protection； b. Horticultural Branch， Liaoning Academy of Agricultural Sciences， Shenyang 110161， China） 　　Abstract： MtDNA COI gene sequences collected from the 7 geographic populations of Spodoptera exigua Hübner in China was used to study the genetic diversity and population expansion in history. The results showed that a total of 8 substitution sites were detected from 206 sequences（sequence length was 547 bp）， identified 5 haplotypes from which including 3 shared hapolotypes and 2 unique hapolotypes. Population genetic diversity was lower（Hd=0.320，π=0.000 70，Kxy=0.383）. There was a certain genetic differentiation （FST=0.398） and little gene flow （Nm=0.378） between different geographic populations. The analysis of molecular variance （AMOVA） showed that the genetic variation come mainly from population， and variation within a population was greater than that between the populations. The neutral test （Tajima’s D=-1.576，P0.05；Fu and Li′s F=-3.834，P0.05） and unimodal mismatch distribution indicated that S. exigua Hübner experienced recent population expansion in North China. 　　Key words： Spodoptera exigua Hübner； COI gene； genetic structure； genetic differentiation 　　甜菜夜蛾（Spodoptera exigua Hübner）属夜蛾科灰夜蛾属，是世界上重要的杂食性农业害虫，可为害玉米、棉花、大豆、甜菜、番茄、白菜及苜蓿等多种作物[1-3]。该种害虫起源于南亚，广泛分布于亚洲、欧洲和北美洲57°N以南的广大地区及整个非洲及