上海市鼠伤寒沙门菌流行特征及分子分型的研究.docVIP

上海市鼠伤寒沙门菌流行特征及分子分型的研究 　　摘要:[目的] 研究上海市2006―2007年鼠伤寒沙门菌腹泻病例株的分子流行病学特征。 　　[方法] 追溯2002―2007年食品中分离的鼠伤寒沙门菌来源,比较鼠伤寒沙门菌食源株与腹泻株的抗生素耐药性;对全球沙门菌监测(GSS)病例和食品中分离的鼠伤寒沙门菌进行血清、耐药表型和脉冲凝胶电泳(PFGE)分子分型。 　　[结果] 经培养确认的鼠伤寒沙门菌腹泻病例数,在2006―2007年本市非伤寒沙门菌型病例构成中仅次于肠炎沙门菌,食品菌株多源于禽(64.4%)和畜肉制品(17.8%),其多重耐药菌株显著高于腹泻株(P0.05)。PFGE将7株食品株和44株腹泻株分为23种带型,优势型为1型(8株)、2型(6株)、29型(4株)、49型(8株)和6型(4株),与食源菌株完全匹配的仅有49型、6型共7个病例(15.9%,7/44)。PFGE-1型腹泻株在2006和2007年分别为2例和6例,2型分别为4例和2例。 　　[结论] 上海市鼠伤寒沙门菌腹泻病例存在高度散发和分散爆发的流行特征,没有与本地食品菌株相匹配的优势克隆型分别是PFGE 2型和1型,与市售生鲜类肉食品污染株之间的遗传同源性低。推测传染源可能由输入性传染源对食品等媒介形成二次污染所致。建议加强流行病学调查,以揭示潜在的传染源和传播途径。 　　关键词: 鼠伤寒沙门菌; 多重耐药; 脉冲凝胶电泳; 分子分型; 传染源 中图分类号:R 117 文献标志码: A 　　 　　Epidemiological characteristics and molecular typing of Salmonellatyphimurium in Shanghai 　　XU Xue-bin1, JIN Hui-min1, XIAO Wen-jia1, CHEN Min1, RAN Lu2, DIAO Bao-wei2, CUI Zhi-gang2, KAN Biao2(1.Shangahi Center of Disease Control and Prevention,Shanghai 200336,China;2.China Center of Disease Control and Prevention,Beijing 100050,China) 　　Abstract: [Objective] To study the molecular epidemiological characteristics of Salmonella typhimurium(S. typhimurium) indiarrhea patients in Shanghai from 2006 to 2007. 　　[Methods] A retrospective analysis from 2006 to 2007 was done to explore the source of food-borne S. typhimurium and make comparison in its drug resistance to antibiotics between food- borne and diarrhea strains. Data of S. typhimurium survey of patients onGlobal-Salm-Surv(GSS) and food were analysed ,including serotype,drug resistance and pulsed field gel electrophoresis(PFGE). 　　[Results] The number of diarrhea cases of S. typhimuriumcomfirmed by culture methodwas onlynextto that ofS. enteritidis in cases of nontyphoidal Salmonella infection in Shanghai between 2006 to 2007. Food-borne S. typhimurium strainswere mostlyfrom poultry(64.4%) andmeat products(17.8%).The antibiotic resistance of food- borne S. typhimuriumwas higher than those from clinical isolateed(P0.05). Sevenfood strainsand 44 diarrheal strainswere d