鲍曼不动杆菌泛耐药性与外排泵基因和外膜蛋白基因相关性的研究临床检验诊断学专业论文.docxVIP

PAGE PAGE IV 鲍曼不动杆菌泛耐药与外排泵基因和外膜蛋白基因相关性的研究 英文摘要 Study on correlation between pan-drug resistance with efflux pump and outer membrane protein in Acinetobacter baumannii Abstract Objective To determine the in vitro activities of 13 antimicrobials including Cefoperazone-sulbactam against clinical isolates of Acinetobacter baumannii and provide experimental evidences for rational use of antimicrobial agents. To investigate the function that induce pan-drug resistance and gene expression of active efflux pump and outer membrane protein in Acinetobacter baumannii. Methods A total of 100 non-repeated strains of Acinetobacter baumannii were isolated from clinical specimens in The First Affiliated Hospital of Suchoow University and all of them had been re-identified by manual test and VITEKⅡCompact of Bio-Merieux. First part The susceptibilities of 100 strains clinical Acinetobacter baumannii was determined by K-B and 2-fold agar dilution method followed by NCCLS recommendations． Second part The genes adeB、adeJ and carO were amplified by PCR and the results were observed by agarose gel electrophoresis．Reverse transcriptase polymerase chain reaction （RT-PCR） was used to measure the mRNA expression levels of active efflux pump AdeABC、AdeIJK . Results First part The antibacterial activity of antibiotic frequently-used is poorer than minocyline、polymixin B and sulperazone. There are 75 pan-drug resistant strains in all 100 strains, their resistant rate to Imipenem 、minocyline、polymixin B and sulperazone are 88%、59.15%、0% and 21.13% while the geometrical mean of MICs are 25.16 （resistance）、5.85（medium）、1.06（sensitive）和 29.3（medium）. Second part The test result of adeB gene including PCR and RT-PCR reveal the differences between resistant strains and sensitive strains have statistical significant while the expression level of adeJ gene between the two groups have no significant difference; 英文摘要 鲍曼不动杆菌泛耐药与外排泵基因和外膜蛋白基因相关性的研究 Th