超声微泡载体系统促进RNAi沉默Survivin基因的实验研究-影像医学与核医学专业论文.docxVIP

第三军 第三军医大学硕士学位论文 PAGE PAGE 1 缩略语表 英文缩写 bp 英文全称 Base Pair 中文全称 碱基对 BSA Albumin from bovine serum 牛血清白蛋白 CCK Cell counting kit 细胞计数试剂盒 d Day 天 DC Duty cycle 占空比 ddH2O DMEM Double distilled water Dulbecco’s modified Eagle’s medium 双蒸水 Dulbecco 改良型 Eagle 培养基 DMSO Dimethyl sulfoxide 二甲基亚砜 FBS Fetal bovine serum 胎牛血清 GFP Green fluorescent protein 绿色荧光蛋白 h Hour 小时 HCC Hepatocellular carcinoma 肝细胞肝癌 Hz Hertz 赫兹 kHz Kilohertz 千赫兹 LB Luria Bertani medium LB 培养基 LM Lipid microbubble 脂质微泡 MB Microbubble 微泡 MHz Megahertz 兆赫兹 MI Mechanical index 机械指数 min Minute 分钟 ml Milliliter 毫升 Mpa Megapascal 兆帕斯卡 nm Nanometer 纳米 OD Optical density 光密度 Opti-MEM Optimal-medium modification Opti-MEM 优化培养基 P Plasmid 质粒 PBS Phosphate-buffered solution 磷酸盐缓冲液 RNAi RNA interference RNA 干扰 rpm Revolutions per minute 转/分钟 s Second 秒 shRNA Small hairpin RNA 短发夹 RNA siRNA Small interference RNA 小干扰 RNA μg Microgram 微克 μl Microliter 微升 UTMD Ultrasound targeted microbubble destruction 超声介导微泡破坏 US W/cm2 Ultrasound Watt per square centimeter 超声 瓦每平方厘米 Ultrasound-Microbubble Vector System Promote RNAi to Silence Survivin Gene Abstract Background: Hepatocellular carcinoma is a kind of deadly cancer with poor prognosis, morbidity and mortality of which rise year after year. Studies have not made significant progresses on seeking effective treatment. Gene therapy as the focus of this field receives great concern by domestic and foreign researchers. Survivin is over-expressed in the majority of tumors as an anti-apoptotic gene which is closely associated to the differentiation, proliferation, invasion and metastasis of tumor cells, considered to be an ideal anti-cancer target. Targeted inhibition of survivin gene expression can weaken the anti-apoptotic ability of tumor cells. RNAi technology is a new tool for gene therapy with low toxicity. It can efficiently block specific gene expression in vivo, inducing gene silencing effect in the process of cell proliferation. siRNA and shRNA are common effective fragm