藏药吉尔巴防治糖尿病视网膜病变药效及其机制研究-药理学专业论文.docxVIP

成都中医药大学硕士学位论文 成都中医药大学硕士学位论文 - - PAGE VIII - Therapeutic effects and mechanism of Berberis Cortex extract on Diabetic Retinopathy Objective ABSTRACT In present study,the effective part extract of a commonly used Tibetan herb Berberis Cortex extract,was selected as the research objiect.Firstly,the acute toxicity test in mice and the determination of some parameters witch related on rat liver and kidney function were conducted to provided scientific basis of safety and effectiveness for clinical use of Berberis Cortex extract.Then we evaluated the regulating effect of blood glucose levels,protection against oxidative stress,protection function on blood vessels and mechanism of DR rats with Berberis Cortex extract. Method Firstly, the acute toxicity test in mice and the determination of some parameters witch related on rat liver and kidney function were conducted to valuated the security of Berberis Cortex extrac; Secondly,the protective effect of pathological changes on retina were observed by utilizing the diabetic retinopathy rats induced by streptozotocin (STZ)； Thirdly, the The regulating effect with blood glucose levels of Berberis Cortex extract were observed by utilizing the following models:the Alloxan induction diabetes mice, diabetic retinopathy rats induced by streptozotocin (STZ) and normal mice; Finally, The NO, MDA and SOD levels of the diabetic retinopathy rats were measured to comment on the regulation of Berberis Cortex extract on oxidative stress. And the HIF, ICAM-1, VEGF, PKC and ANGⅡlevels of the diabetic retinopathy rats were measured to evaluate the vascular protection mechanism of Berberis Cortex extract . Results Firstly,according to the results of the acute toxicity test in mice,the median lethal dose of Berbreis Cortex extract on mice was 17.169 g·kg-1,equivalent to 206 times of clinical dosage respectively.The liver and kidney function of rats were not damaged by Berberis Cortex extract. Secondly, Berberis Cortex extract could