超临界二氧化碳抗溶剂法制备玉米蛋白基纳米营养物-化学工程与技术专业论文.docxVIP

摘要 摘要 - - II - 合可制备不同粒径及分布要求的微粒。热重分析和差热分析的结果显 示制得的玉米蛋白空白微球较原料稳定性有所提高。 叶黄素-玉米蛋白纳米胶囊的制备和表征。在玉米蛋白空白微球制 备的基础上，选用叶黄素作为模型药物，采用SEDS法制备叶黄素-玉米 蛋白纳米胶囊。考察了温度、压力、溶液浓度、芯材比和溶液流速对 叶黄素纳米胶囊形貌、粒径、载药量和包埋率的影响。用扫描电镜、 纳米粒度分析、紫外-可见光谱分析、差热分析、X射线衍射分析等手 段对结果进行表征。实验结果表明，SEDS法可制备出较高载药量和包 埋率的叶黄素-玉米蛋白纳米胶囊，温度、压力、芯材比、溶液流速都 会影响纳米胶囊的粒径、包埋率和载药量。在温度为45℃、压力为 10MPa 、玉米蛋白溶液浓度为 1.5% 、芯材比为 1 ： 18 、溶液流速为 1mL/min的最佳条件下制备出的叶黄素纳米胶囊载药量有5.87%，包埋 率为60.38%，此时纳米胶囊的平均粒径为205nm。差热分析和X射线衍 射分析的结果表明，纳米胶囊中叶黄素以晶体的形式存在，且叶黄素 晶体是被包埋在玉米蛋白中形成叶黄素-玉米蛋白纳米胶囊。 最后对全文作了总结，对文章的创新点和不足做了概括并提出后 续发展的建议。 关键词：超临界二氧化碳，超临界抗溶剂法，玉米蛋白，纳米颗粒， 叶黄素，纳米胶囊，纳米营养物 Abs Abstract - - III - PREPARATION OF NANO-NUTRACEUTICALS BASED ON ZEIN BY SUPERCRITICAL CO2 ANTISOLVENT PRECIPITATION ABSTRACT Zein is a natural biological macromolecules, with excellent biocompatible and biodegradable properties. It has already been reported as a sustained release drug carrier material, most of which are zein microspheres. Zein microspheres which were used to encapsulate, protect, and deliver active ingredients had good sustain-release properties, and took on good anti-pepsin decomposition characteristics. So zein is a good enteric coating material. And as natural biomacromolecules, food proteins are generally considered to be biocompatible and biodegradable. Further more, they are GRAS (generally regarded as safe) ingredients and have high nutritional value. Using supercritical CO2 to encapsulate bioactive substances is a newly developed method and has been widely used because of low critical temperature, pressure and no toxicity. Supercritical CO2 Antisolvent Precipitation(SAS), with its more feasible applications, has been given more attention. In this study, the drug carries based on zein was prepared using solution enhanced dispersion by supercritical fluids (SEDS), and its application in encapsulating lutein to prepare lutein/zein nanocapsules was investigated. Firstly, the preparation and the characterization of the zein nanoparticles. Zein nanoparticles was prepared f