草原龙胆的胚胎学研究-园林植物与观赏园艺专业论文.docxVIP

东北林业人学硕．1：学位论文Abstract 东北林业人学硕．1：学位论文 Abstract E淞，om口g厂口门d矽D，．“m gro、Vn as a commercial cut flowerhas hi曲ly decorative characteristics with a Variety of flower color and form．It’s a good material for embryological study since there are 2000 to 3000 oVules in an oVary．This research proVides basic info肌ation f-or genetics and breeding of this plant．A breeding line“02．49’’of E j呈阳门cZ沙D，“，”with yellow single flowers was used because it was almost genetically pure due to self二fenilization for many years，a nowering period was long and heredita巧traits were stable．In December of 2003 and 2004，seeds were sown in a greenhouse kept at 7 to l 50C at Research Institute of Flower Biotechnology，Nonheast Forestry UniVersity．Next year 500 seedlings were chosen and replanted for growth．Flowers were sampled from plants 60cm high in July to August when temperature in a greenhouse was 1 5—30℃．More than 500 nower buds or flowers on the second or third lateral branches were taken at l 0 gro、Vth stages ranging f}om 0．2 to 4cm in bud length．Microsporogenesis，megasporogenesis，and the development of the male and female ganletophytes were obser、7ed nlicroscopically．ARer manual nower polIination，5 to 1 O styles and oVaI．ies on Ihe second or third lateral branches were taken at dif．ferent deVelopnlental stages：at interVal of 2 hoLlrs during nrst 48 hours aRer pollination，at interval of 4 hours for next 48 hours，and at 2 or 3一day interVal thereaf!ter to 60 days at、ter pollination．Fertilization process．and the deVeIopment of the embryo and endospernl were observed microscopically． Materials were 6xed in FAA(5 mL formalin： 6 mL acetic acid： 89 mL 70％ethan01)． After stained with Ehrnch’s haematoxylin，the nxed tissues were embedded in para衢n using conVentional nlethod．and cLlt at a thickness of 6 to 1 O um．More than 2000 slices were prepared with 1 0 to 20 continLlous sections each slice．ObserVation and photomicroscopy of scc【ions、Vere carried oLIt usillg lighI nlicroscope(OlynlpLls．BX 1 5)． T11e