枸杞多糖提取条件优化及体外抗氧化活性的研究.docVIP

枸杞多糖提取条件优化及体外抗氧化活性的研究 　　摘要：为了优化枸杞（Lycium chinense）中多糖的提取工艺并研究其抗氧化活性，以宁夏枸杞为原料，以水提醇沉为提取方法来研究其提取工艺。通过单因素试验，考察不同料液比、浸提温度、浸提时间、浸提pH、提取次数、醇沉时间、醇沉时乙醇的加入量等诸多因素来优化枸杞多糖的提取率；通过抗脂质过氧化能力的测定以及对超氧阴离子自由基清除作用的测定对枸杞多糖的抗氧化能力进行研究。结果表明，在提取枸杞多糖时，料液比为1∶35（g∶mL），温度为90 ℃，pH为11，浸提3 h，提取2次，合并提取液，加3倍体积95%乙醇沉淀6 h时，提取效果最佳，提取率为18.56%。另外，在提取时，采用超声波辅助提取30 min，可提高多糖提取率。抗氧化结果显示，枸杞多糖具有一定的抗氧化活性。 　　关键词：枸杞（Lycium chinense）多糖；提取；抗氧化活性 　　中图分类号：TS201.1；S567.1+9 文献标识码：A 文章编号：0439-8114（2015）06-1440-05 　　DOI：10.14088/j.cnki.issn0439-8114.2015.06.040 　　Abstract：To optimize the process of extracting the polysaccharides from Chinese wolfberry and study its anti-oxidant， the ningxia wolfberry was sinked in water and extracted with alcohol method. Through single factor experiment， the different material liquid ratio， extraction temperature， extraction time， extraction of pH， extraction times and alcohol sink time， alcohol sink when， the amount of ethanol and extraction of auxiliary raw materials extracted in a different way， and many other factors were optimized. The single factor experiment， L9（34） orthogonal experiment and variance analysis was integrated. The phenol sulfuric acid method was used to measure polysaccharide yield. The effects of Lipid peroxidation and radical scavenging on superoxide were studied for testing the anti-oxidant activity. Results showed that the optimal LBP extraction conditions were the ratio of material to liquid of 1∶35（g∶mL）， 90 ℃， pH 11， 3 h leaching， extracting 2 times， merging the extracted liquid， adding 3 times 95% ethanol precipitation at 6 h. The extraction rate was 18.56% under these conditions. In addition， microwave assisted extraction for 30 min improved the polysaccharides yield. The Chinese wolfberry polysaccharides had good anti-oxidant activity. 　　Key words： Chinese wolfberry（Lycium chinense） polysaccharides ；extraction； anti-oxidant activity 　　枸杞（Lycium chinense）为茄科（Solanaceae）植物，是落叶小灌木，可食用及药用的成熟果实被称为枸杞或枸杞子。枸杞在全国各地均有野生，以宁夏、河北、甘肃及云南等地较多。宁夏产的皮薄肉厚，色泽艳红，质量优良，久负盛