促性腺激素释放激素激动剂对体外培养子宫肌瘤肌细胞及内膜细胞凋亡影响-妇产科学专业论文.docxVIP

Up-regulation of apoptosis by gonadotropin-releasing hormone agonist in cultured myomatous cell and endometrial cell of symptomatic uterine myomas Postgraduate: Wang Dongyu Supervisor: Prof. Kang Jiali Guangzhou First Municipal People’ s Hospital, The Affiliated Hospital of Guangzhou Medical College Abstract Objective 1. To investigate the effects of gonadotropin releasing hormone agonist (GnRHa) on apoptosis of cultured myomatous cell and endometrial cell. 2. To investigate the relationship between apoptosis and uterine myoma.To evalute the relationship between apoptosis of cultured endometrial cell and abnormal uterine bleeding. To further explore the mechanism of GnRHa in the treatment on uterine myoma and provide new approach and experimental evidences for the clinical treatment of uterine myoma. Materials and methods 1. Samples From Jan 2008 to June 2009, uterine myomatous cells(study group A)， myometrial cells (control group A)and endometrial cells(study group B) from 36 women(mean age 38.21 ± 2.28, range 28-46) who received myomectomy for uterine myomas with abnormal uterine bleeding and endometrial cells (control group B) from 22 women(mean age 35.23 ± 3.32, range 22-45) who received ovarian cystectomy surgery for benigh ovarian epithelial tumor hospitalized in gynaecologic department were cultured in vitro. Biopsy specimens were obtained from all subjects in the proliferative phase by endometrial curettage before surgery. Endometrial phase was assigned on the basis of the histologic evaluation and subjects with endometrial hyperplasia or endometrial carcinoma were excluded.All the patients were without any other gynecologic diseases, such as endometriosis, and had not received any hormonal medical treatments during the prior 3 months. 2. Methods Levels of apoptosis were examined in myomatous cells, myometrial cells and endometrial cells before and after inc