二氧化硅壳聚糖修饰茶多酚纳米脂质体的结构和生物利用性-水产品加工及贮藏工程专业论文.docxVIP

ABSTRACT Liposome as a novel delivery system, has been widely applied in pharmaceutical, cosmetical and food industry. However, it suffers a lot of problems including self-aggregates, easy drug leakage from the system, which severely limits the application of liposome. Nowadays, researchers proposed modification of liposome through several ways to improve the properties of liposome, such as surface modification by sediment of polyelectrolyte outside the liposome, preparing innovative liposomes with new characteristics by lipid derivatives, etc. In this paper, tea polyphenol (with great bioactivity, embracing anti-oxidation, anticancer, anti-inflammatory, etc. what’s more, it is easy to be detected) was chosen as the core material. Tea polyphenol nanoliposome(TPN) was obtained by combining ethanol injection with dynamic high-pressure microfluidization method. Through following modification, the traditional modification liposome chitosan-liposome(CH-TPN) was obtained by chitosan. Furthermore, based on CH-TPN, a new type of modified liposome silica-chitosan-liposome(S-CH-TPN) was acquired. Further investigation on the modification theory was conducted, along with the effect of modification material on the structure as well as the bioavailability of tea polyphenol liposome. TPN showed an average size of 101.3±1.49nm, zeta value of -10.59±0.35mV, and the modified samples CH-TPN, S-CH-TPN based on it exhibited an average diameter about 231.83±0.57nm, 573.6±2.89nm, respectively, and zeta value of 12.65±0.04 mV, 7.82±0.28 mV, respectively. All of the three samples displayed small PDI value, which means uniformity system. The determination of size and zeta confirmed the successful coat of modification layer outside the liposome. Further analysis about interaction mechanism between modification materials and lipsome was showed, that CH-TPN embraces fluffy appearance with chitosan coat on the liposome like patches, meanwhile, the hydrophobic structure of chitosan inserted int