超高效液相色谱-食品安全质量检测学报.PDF

7 7 Vol. 7 No. 7 2016 7 Journal of Food Safety and Quality Jul. , 2016 - 19 1 2 甦1 2 2 1* 胡 青 , 林 媛 , 张 , 郑希望 , 崔亚君 , 季 申 (1. , 201203; 2. , 201203) 摘 要: 目的 - 方法 -0.1%( V:V=3:2)3 , , WAX WCX , -结果 19 62.3%128.2%, 0.5 345.5 µg/kg, 结论 , 关键词: ; ; ; - Detection of 19 dark pigments illegally added in Mumu Fructus by ultra high performance liquid chromatography-tandem mass spectrometry 1 2 1 2 2 1* HU Qing , LIN Yuan , ZHANG Su , ZHENG Xi-Wang , CUI Ya-Jun , JI Shen (1. Shanghai Institute for Food and Drug Control, Shanghai 201203, China; 2. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China) ABSTRACT: Objective To establish a method for detection of 19 dark pigments illegally added in Mumu Fructus by ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Methods Samples were extracted with methanol-0.1% formic acid solution ( V:V=3:2) by ultrasonic 3 times, cleaned up with WAX and WCX columns to obtain acidic and alkaline pigments tested solution, respectively. Finally, the analytes were detected by UHPLC-MS/MS. Results Recoveries of 19 kinds of dark pigments in Mumu Fructus were 62.3%128.2%, and the limits of detection were 0.5345.5 µg/kg. This method could be applied