不同分期肺癌组织中Beclin1p53p16表达情况剖析.docVIP

不同分期肺癌组织中Beclin1p53p16表达情况剖析 　　[摘要] 目的 本文旨在通过对肺癌组织中Beclin1、p53和p16表达的分析,为肺癌早期诊断及其恶性度评估提供可靠数据。方法 选择2009年1～12月在山西省汾阳医院就诊的肺癌患者,取肺组织50例,检测样本中不同分期肺癌组织Beclin1、p53、p16的表达情况,对该表达情况与对应的分期进行方差分析与LSD-t检验。结果 不同肺癌分期的Beclin1、p53、p16的表达情况之间的差别有统计学意义(P0.05)。结论 肺癌各临床分期的Beclin1、p53、p16表达情况不一样。随肺癌分期的增高p53表达量增高,p16表达量下调,Beclin1表达量降低。 　　[关键词] 肺癌; Beclin1; p53; p16 　　[中图分类号] R734.2 [文献标识码] A [文章编号] 1673-9701(2010)07-26-02 　　 　　Expression of Beclinl,p53 and p16 in Different Stages of Lung Cancer 　　LI Xiaoyan1 WANG Guiqin2 　　1.Affiliated Fenyang Hospital of Shanxi Medical University,Fenyang 032200,China;2.Teaching and Research Section of Microbiology and Immunology,Shanxi Medical University,Taiyuan 030001,China 　　 　　[Abstract] ObjectiveTo investigate expression of Beclinl,p53 and p16 in lung cancer tissue of different stages and provide reliable data for early diagnosis of lung cancer and the assessment of its m alignant degree. MethodsWe selected 50 patients with lung cancer from Shanxi Fengyang Hospital between Jan.2009 and Dec.2009. The expression levels of tissue Beclinl,p53 and p16 in different stages of lung cancer were detected and the analysis of variance and LSD-t test were used for their expression and their corresponding stage. ResultsThere were significant differences in the expression levels of Beclinl,p53 and p16 in different stages. ConclusionThe expression levels of Beclin1,p53 and p16 in the tissue of lung cancer in different clinical stages are different. With the increase in staging of lung cancer,the p53 expression level is increased,the p16 expression level is down-regulated and the Beclin1 expression level is decreased. 　　[Key words]Lung cancer; Beclin1; p53; p16 　　 　　肺癌是世界上发病率和死亡率最高的恶性肿瘤之一。多数患者在确诊时已失去根治的机会,早期发现和诊断肺癌及其恶性度的评估对其治疗和预后都有重要意义。肺癌早期诊断与恶性度评估分子标志物的筛选是临床肿瘤工作者亟待解决的课题[1,2]。 　　自噬现象是一种高度保守的细胞行为,存于所有的物种。研究表明Beclin1是唯一发现的哺乳动物的自噬基因,是自噬的执行者;又是一类新的抑癌基因,只有在双等位染色体都表达时才发挥抑癌作用。Beclin1基因的缺失性突变导致自发性肿瘤发生率提高。Beclin1基因表达异常(减少或缺失)是否为肿瘤细胞早期事件?如果研究结果是肯定的,那么我们可以将其作为早期诊