黄皮桑寄生不同极性溶剂提取物抗氧化能力的研究.docVIP

黄皮桑寄生不同极性溶剂提取物抗氧化能力的研究 　　摘要:试验采用清除DPPH自由基#65380;清除ABTS+自由基和还原能力3种体外抗氧化测定方法,对寄主为黄皮树[Clausena lansium (Lour.) Skeels]的桑寄生(Taxillus chinensis)不同极性溶剂提取物的抗氧化能力进行了研究#65377;结果表明,黄皮桑寄生乙酸乙酯和正丁醇提取物具有较优异的抗氧化能力,其结果与其有较高的总黄酮含量有关#65377;说明黄皮桑寄生乙酸乙酯和正丁醇提取物具有较好的开发天然抗氧化剂的潜力#65377; 　　关键词:桑寄生(Taxillus chinensis);黄皮[Clausena lansium(Lour.) Skeels];抗氧化;寄主 　　中图分类号:R284.2;Q946文献标识码:A文章编号:0439-8114(2014)11-2631-03 　　 　　Antioxidant Activity of The Extracts from Taxillus chinensis Parasitized 　　on Clausena lansium (Lour.) Skeels 　　 　　HUO Li-ni1,2,CHEN Rui3,LIAO Yan-fang4,LIU Hua-gang1,HUANG Mao-chun3,HU Yue3 　　(1.College of Pharmacy, Guangxi Medical University, Nanning 530021, China; 2.College of Pharmacy,Guangxi Traditional Chinese Medical University, Nanning 530001, China; 3.College of Chinese Medical Science, Guangxi Traditional Chinese Medical University, Nanning 530022, China; 4.Guangxi Institute of Chemical Industry, Nanning 530001, China) 　　 　　Abstract: Three in vitro antioxidant methods such as DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS+ (2,2＇-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) scavenging assay and reducing assay were used to study the antioxidant ability of the petroleum ether, ethyl acetate and n-butanol extracts from Taxillus chinensis parasitized on Clausena lansium (Lour.) Skeels. The total flavonoids(TF) in all extracts were determined by UV methods. The results showed that the ethyl acetate extracts(EE) and n-butanol extracts(BE) had better antioxidant ability which might related with its high content of total flavonoids. It is indicated that EE and BE have good development potential for natural antioxidant. 　　Key words: Taxillus chinensis; Clausena lansium (Lour.) Skeels; antioxidant; host 　　 　　基金项目:广西教育厅课题项目(2013YB137;2013LX063);广西卫生厅中医药民族医药继承创新工程课题项目(GZZC13-01;GZZC13-02) 　　 　　中药桑寄生为桑寄生科钝果寄生属植物桑寄生(Taxillus chinensis)的干燥带叶茎枝,主产于广东#65380;广西#65380;云南#65380;福建等地,具有补肝肾#65380;强筋骨#65380;祛风湿#65380;安胎元等功效,用于治疗风湿痹痛#65380;腰膝酸软#65380;筋骨无力#65380;崩漏经多#6