黄精种子萌发抑制物质及其去除的方法的研究初探.docVIP

黄精种子萌发抑制物质及其去除的方法的研究初探 　　摘 要：为探讨黄精种子抑制物质生理活性及种子中抑制物质的去除方法，采用乙酸乙酯、甲醇和水提取对黄精种子进行浸提，并测定提取物对白菜幼苗生长的抑制活性；用温水浸种12，24，36 h，测定各处理黄精种子萌发率。结果表明，黄精种子浸提液（乙酸乙酯提取液除外）对白菜幼根及胚轴均有显著抑制作用，且抑制物质的活性随着提取物浓度的增加而增强，其中水提取液的抑制活性高于甲醇提取液，黄精种子乙酸乙酯提取液对白菜幼苗无显著抑制作用（P0.05）；温水浸种时间对黄精种子萌发时间及萌发率均产生显著影响，其中温水浸种24 h的种子，沙藏30 d种子完成萌发，萌发率达91%，而温水浸种12 h和36 h处理组的种子，沙藏40 d种子完成萌发，萌发率分别为89%和79%。综合分析表明，黄精种子中存在抑制物质，用温水浸泡可有效除去种子抑制物质，以浸种24 h最佳。 　　关键词：黄精；种子休眠；抑制物质 　　中图分类号：S567.23+9 文献标识码：A DOI 编码：10.3969/j.issn.1006-6500.2018.03.005 　　Abstract： In order to study the bioassay of endogenous germination inhibitors and the removal method of inhibitors in Polygonatum sibiricum seeds， the P. sibiricum seeds were extracted by ethyl acetate， methanol and water， the effect of solvent extracts on Chinese cabbage seedlings was measured； the P. sibiricum seeds were presoaked 12， 24， 36 hours with the warm water， then the germination rate of each treatment was measured.The results showed that the extracts （except ethyl acetate extract） of P. sibiricum seeds had significant inhibitory effects on hypocotyl and radicle length of Chinese cabbage seedlings， and the inhibitory ratio was increased with the increase of extract concentration. The inhibitory effect of water extract was significant higher than methanol extract， and the ethyl acetate extract had no inhibition at any concentration（P0.05）. The germination time and germination rate of P. sibiricum seeds were greatly influenced by the different soaking time of warm water. When the seeds were presoaked 24 h， the germination completed at 30 days in sand storage， and the germination rate was 91%. When the seeds were presoaked 12 h and 36 h ， the germination completed at 40 days， and the germination rates were 89% and 79%， respectively. These results suggested that there were inhibitory substances in P. sibiricum seeds and the inhibitors could be removed effectively by soaking with the warm water， and the optimum presoaked time was 24 h. 　　Key words： Polygonatum sibiricum； see