丙泊酚对兔脊髓缺血再灌注损伤的保护作用.docVIP

丙泊酚对兔脊髓缺血一再灌注损伤的保护作用 吕苗苗孙绪德高昌俊柴伟张贵和赵辉张振 】目的：观察丙泊酚脊髓组织中低氧诱导因子一 1 (HIF-la )的变化以及 神经行为学和脊髓组织病理学改变，探讨丙泊酚对脊髓缺血再灌注损伤的保护作 用。方法：60只新丙兰大白兔随机分为三组：A组(缺血-再灌注组)，8组(缺 血-再灌注+英脱利匹特组)，C组(缺血-再灌注+丙泊酚组)。采用球囊压迫法 建立兔脊髓缺血-再灌注模型。采用Tarlov评分法评价兔复灌后48h神经行为学 功能，显微镜下观察14、6脊髓组织的病理生理学改变，采用免疫组化法监测复灌 后48小时、72小时及1周HIF-la的变化。结果：C组48h神经行为学Tarlov 评分［(3.0±1.3)分］明显高于 A 组［(1.0±1.2)分］和 B 组［(1.0±1.1)分］(P0.05)， A组与B组差别无统计学意议。神经元计数C组(8.5土3.5)显著高于A组(2.3 ±2.1)和B组(2.2.±2.0)。C组HIF-1 o表达较其他两组明显增加(P 0.05)。结 论：丙泊酚能增加脊髓组织HIF-la的表达，可能促进下游靶基因的表达，使受 损组织的血管再生与重建，从而发挥对脊髓缺血-再灌注损伤的保护作用。 关健词】丙泊酚；HIF-la;脊髓；缺血-再灌注损伤 Effects of Propofol on the Expression of Hypoxia Inducible Factor-1 a in Ischemia-reperfusion Injury of Spinal Cord LV Miaomiao，SUN Xu-de,GAO Chang-jun, ZHANG Gui-he，et al.Department of Anesthesiology, Tangdou Hospital, Fourth Military Medical University, Xian.710038?China Abstract】 Objective To investigate the effection on protection of propofol against spinal cord ischemia-reperfusion Injury (IRI) and the effects of propofol on the expression of hypoxia 基金项A:陕西省科技攻关项R (批准号：2005K12-G5(12)) 单位：710038丙安市，第四军医大学唐都医院麻醉科 责任 :孙绪德 inducible factor-1 o (HIF-1 a )in spinal cord IRI. Methods:Sixty New Zealand white rabbits were devided to three groups: (A) IRI group; (B) IRI and intralipid group; (C) propofol and IRI group. Spinal cord ischemia was accomplished in rabbits by occlusion of the infrarenal aorta with a balloon catheter for 30 minutes. Neurological behavior was assessed according to Tarlov scoring system at 48 h of reperfusion. The spinal cord lumbar segment (L4-6) of was resected at 48 h of reperfusion for microscopic examination. The total number of normal motor neurons in the anterior horn was counted and collect average number. The expression of HIF-1 a was detected by immunohistochemistry analysis in spinal cord tissue. Results The Tarlov scores of group C (3.0± 1.316) is higher than of group A(L0 土 1.363) and group B (1.0 ± 1.21 l )(P0.05).Thc numbers of normal motor neurons in the anteri