海藻糖联合磷酸盐冷冻保存血小板的实验-研究.pdfVIP

DMSO及2％DMSO联合TS相同，维持血小板低渗休克反应、聚集功能及抑制 血小板凋亡的效果优于6％DMSO及2％DMSO联合TS，并能较好地维持血小板 的超微结构。 关键词 海藻糖 血小板 冷冻保存 第3页 Platelet acombinationof cryopreservation using trehaloseand as phosphatecryoprotectants ABSTRACT aimofthis wastoobservethe functionoftrehalose Objeetive：Thestudy protective and at-80Conhuman torealizethe apheresis phosphate platelets，and long-term(3 fonctionofthis solution． months)protective plateletstorage Methods：Human weretreatedwithtrehalose apheresis platelets plusphosphate．6％ DMSOand2％DMSOTS．then frozenforl plus placedin·80℃refrigerator．After were monthand3 thaw戗Iand week,l months，samples included shock induced plateletcount,hypotonieresponse，ADP andultrastrueture ofplatelets． rateof beforeandafter trchalosewere Results：(1)Theapoptosisplatelets loading rateof (1．3士o．5)％and(1．9±1．1m‘》O．05)，respectively．(2)Theapoptosisplatelets with treated trehalose(without lachalose w∽ phostphate)andplusphosphate countof (11．6-a：3．9)％and(6．44-0．9m妒o．05)，respectively．(3)Theplatelet platelets treatedwithtrehalose forl monthand3monthswele