大豆抗原蛋白对断奶仔猪血清中细胞因子的影响及肠道致敏作用-临床兽医学专业论文.docx

奶仔猪回肠紧密连接蛋白 Claudin-1 表达量（P＜0.01）。 4、β-伴大豆球蛋白和大豆球蛋白能够不同程度地降低仔猪肠道紧密连接蛋白 Claudin-1 mRNA 相对表达量。添加 2000 μg/kg·W、5000 μg/kg·W 剂量的 7S 和 11S 能够显著降低仔猪十二指肠紧密连接蛋白 Claudin-1 mRNA 相对表达量（P＜0.05 或 P ＜0.01），添加 500 μg/kg·W、2000 μg/kg·W、5000 μg/kg·W 剂量的 7S 和 11S 能够显 著降低仔猪空肠前段紧密连接蛋白 Claudin-1 mRNA 相对表达量（P＜0.05 或 P＜ 0.01），添加 5000 μg/kg·W 剂量的 7S 和 11S 能够显著降低空肠中段、空肠后段和回 肠紧密连接蛋白 Claudin-1 mRNA 相对表达量。 综上所述，β-伴大豆球蛋白和大豆球蛋白能够明显提高仔猪血清中 IL-2、IL-4、 IL-6、IL-8 含量，降低 INF-γ含量；饲喂大豆抗原蛋白后的仔猪小肠各段绒毛高度降 低、隐窝深度增加，肠黏膜紧密连接蛋白 Claudin-1 及其 mRNA 表达量降低。 关键词：β-伴大豆球蛋白，大豆球蛋白，紧密连接，Claudin-1 II Abstract The allergization model was established by adding different levels of purified β-conglycinin (7S) and Glycinin (11S) to diets of weaned piglets. Cytokines, intestinal mucosal morphology, expression of tight junctions protein Claudin-1 and its genes expression levels were studied by the methods of ELISA, paraffin sections, immunohistochemistry and real-time fluorescence quantitative PCR (qRT-PCR), respectively, in order to explore the allergization of 7S and 11S in weaned piglets. Seventy crossbred weaned piglets (Duroc×Landrace×Yorkshire), 24 days old, were selected and allotted to seven groups randomly, ten piglets in each goup . All of the piglets were fed with milk replacer at the age of 17 day, weighed and weaned at day 24 , then started to feed with experimental diets. The piglets in the control group (A) were fed with basic diet without soybean antigen protein. The piglets in Group B, Group C, and Group D were fed with the basic diet with 7S at 500 μg/kg·W, 2000 μg/kg·W, and 5000 μg/kg·W , respectively. The piglets in Group E, Group F, and Group G were fed with the basic diet with 11S at 500 μg/kg·W, 2000 μg/kg·W, and 5000 μg/kg·W, respectively. Blood samples were collected from the anterior vena cava at days 26, 28 and 30 for measuring cytokines (IL-2, IL-4, IL-6, IL-8, and INF-γ) in sera. At the age of 31 day, five piglets of each group were slaughtered. Segments of duodenum, mid-jejunum and ileum were colle