雌激素在新生期结直肠炎症诱导的成年大鼠慢性内脏痛过敏中的作用及其机制的研究消化病学专业论文.docxVIP

PAGE PAGE IV 雌激素在新生期结直肠炎症诱导的成年大鼠慢性内脏痛过敏中的作用及其机制的研究 英文摘要 Activation of estrogen signaling contributes to P2X receptor sensitization in chronic visceral hyperalgesia in adult female rats induced by neonatal colonic inflammation Abstract Aims Chronic abdominal pain and cramping in patients with irritable bowel syndrome （IBS） remains a big challenge for clinicians. IBS is 2~3 times more prevalent in women than in men. However, the precise mechanism remains unknown. The aim of this study was designed to investigate whether estrogen signaling is involved in visceral hypersensitivity and whether estrogen enhances expression of P2X3 and CBS receptors in colon-specific dorsal root ganglion （DRG） in a rat model of neonatal colonic inflammation （NCI）. Methods 1, Visceral hyperalgesia was induced by colonic injection of 0.5% acetic acid （AA） in 10-day-old female rats and ovariectomized （OVX） experiments were conducted when rats grown to adult. 2, Behavioral response to colorectal distension （ CRD ） was measured by visual observation of abdominal withdrawal reflex （AWR）. 3, Colon specific DRGs（T13-L2）were analyzed for P2X3 and CBS receptor expression by western blotting. 4, Estrogen was incubated with colon specific DRGs （T13-L2） then the expression of P2X3 and CBS receptor was measured. Results 1, NCI significantly increased the visceromoter response to CRD at 20, 40, 60 and 80 mmHg. The increased response lasted from 7 weeks to 11 weeks （*p0.05）and disappeared at 12 weeks（p0.05） within our observation time period. 英文摘要 雌激素在新生期结直肠炎症诱导的成年大鼠慢性内脏痛过敏中的作用及其机制的研究 2, OVX significantly decreased AWR scores when compared with these rats with neonatal AA infusion before surgery. OVX produced no obvious effect on rats received normal saline injection when they were 10-day-old. After OVX, some rats were treated with 17β-estradiol injection（20ug/kg）（OVX +E2）. AWR scores were significantly increased two weeks after E2 treatment. 3, CBS and P2X3 expression was m