成体大鼠动脉炎时血管身份分子标志发生重塑-外科学(普通外科)专业论文.docxVIP

成体大鼠动脉炎时血管身份分子标志发生重塑 硕士研究生：曹龙磊 导 师： 吕 平 教授 中文摘要 目的：研究成体大鼠动脉炎时血管身份分子标志是否发生重塑，同时观察动脉炎 血管壁结构成分的重塑情况。 方法： 取 7 只 10-12 周龄成年雄性 Wistar 大鼠为实验研究对象。全部大鼠右颈动 脉周围放置聚乙烯管制作动脉炎作为实验组，全部大鼠左颈动脉只解剖不 制作动脉炎作为对照组。其中 3 只大鼠左，右颈动脉采用 RT-PCR 法检测 Ephrin-B2， Eph-B4，IL-1，α-SMA，Elastin, NF-KB 和 Collagen 1 基因的 表达。另外 4 只大鼠左，右颈动脉石蜡包埋制作切片后进行 HE 染色及 Masson 染色。采集图片后使用电脑图像分析仪测定血管管壁横截面的内膜 +中膜厚度以及外膜厚度，用 image pro plus 6.0 软件对颈动脉胶原蛋白阳 性表达进行累计光密度测定。数据用 mean ± SEM 表示，两组数据的统计 分析比较采用配对 t 检验，P 0.05 为显著性差异。 结果：1 实验组 NF-KB，IL-1，α-SMA，Elastin，Collagen 1，Ephrin-B2 和 Eph-B4 的表达均高于对照组（ P 0.05）。 2 与对照组相比较，实验组中颈动脉内膜+中膜厚度、外膜厚度均显著增加 （ P 0.05）。 3 与对照组相比，实验组中胶原蛋白含量增多，胶原纤维增生（P 0.05）。 结论：成体大鼠动脉炎时，在炎性信号通路相关基因表达增加的同时，动脉血管壁结 构成分基因和血管身份分子标志 Ephrin-B2 及 Eph-B4 基因的表达也显著增加， 说明成体大鼠动脉炎时不仅血管壁结构成分发生重塑，血管身份分子标志也发 生了重塑。 关键词：动脉炎；血管身份；Ephrin-B2；Eph-B4；重塑 Molecular markers of vessel identity are remodeled during arterial inflammation in adult rats Master Candidate : Longlei Cao Supervisor : Prof. Ping Lü Abstract Objective : To examine whether the molecular markers of vessel identity are remodeled, and observe the remodeling of structure and composition of vessel wall during arterial inflammation in adults rats. Methods : Seven 10- to 12-week-old male Wistar rats were used in this study. The right carotid arteries of all rats were wrapped by polyethylene tubes (4-mm long PE-90) to provoke arterial inflammation and assigned to experimental group. The left carotid arteries of all rats were sham operated and assigned to control group. The left and right carotid arteries of three Wistar rats were tested by RT-PCR to examine the expression levels of NF-KB, IL-1, α-SMA, Elastin, Collagen I, Ephrin-B2 and Eph-B4 genes. The left and right carotid arteries of the other four rats were embedded in paraffin and cut in cross section for Hematoxylin eosin (HE) and Masson trichrome staining. All images were acquired. The intima + media and adventitia thicknesses of vessel cross-sections were measured using image analysis, and the intensity of collagen