丹参川芎嗪注射液对大鼠坐骨神经缺血再灌注损伤的影响及其机制-麻醉学专业论文.docx

PAGE PAGE 10 丹参川芎嗪注射液对大鼠坐骨神经缺血再灌注损伤 的影响及其机制 中文摘要 目的：通过建立 SD 大鼠肢体缺血再灌注模型，观察丹参川芎嗪注射液对缺血再 灌注坐骨神经的保护作用，并探讨其可能的影响机制。 材料与方法：选择雄性SD大鼠30只，随机分成对照组（C组）、模型组（I1，I2 组）、给药组（T1，T2组），每组6只。对照组经腹腔注射10%GS半小时后分离 左髂总动脉不结扎，5小时后描记步行轨迹并取左侧坐骨神经和提取血清；模型 组、给药组予无损伤外科动脉夹阻断大鼠左髂总动脉使左后肢缺血3小时后松开 动脉夹，恢复血流2小时后I1组和T1组取左坐骨神经及提取血清。左坐骨神经标 本行HE染色病理、免疫组化染色NF-κB表达、超微结构变化观察；血清行血清 酶LDH、MDA、SOD、GSH-Px检测；对I2组T2组在缺血3小时再灌注2小时即刻， 1W、2W、4W测量大鼠步行轨迹并分析其坐骨神经功能指数（SFI）。 结果：成功建立缺血再灌注模型。缺血再灌注（IR）后，T组和I组的乳酸脱氢酶 （LDH）、丙二醛（MDA）均较对照组增高，T组的增高幅度小于I组，均具有统 计学意义（P0.05）；T组的氧化物歧化酶（SOD）及谷胱甘肽过氧化物酶（GSH-Px） 则高于I组，具有统计学意义（P0.05）；T组大鼠神经组织中NF-κB阳性表达细 胞数少于I组，具有统计学意义（P0.05）；T组的超微结构变化优于I组；T组的 坐骨神经功能指数（SFI）在缺血再灌注后1W、2W、4W均明显优于I组，具有 统计学意义（P0.05）。 结论：周围神经存在缺血再灌注损伤；丹参川芎嗪注射液对周围神经缺血再灌注 具有保护作用。 关键词；缺血再灌注，坐骨神经，丹参川芎嗪 The effects of Salvia Ligustrazine Injection on sciatic nerve ischemia- reperfusion injury in rats and its possible mechanism Abstract Objective: To observe the protective effects of Salvia Ligustrazine injection on sciatic nerve ischemia-reperfusion (IR) injury in rats, and explore its possible influencing mechanism. Methods: 30 male SD rats were randomly divided into compared group, model group (I1, I2) and HYPERLINK /medication_administration_team medication group (T1, T2) equally (6 per group). Their ischemia-reperfusion models were Installed by the following steps: The left common iliac arteries of group C were separated but non-ligated after being injected of 10% GS by intraperitoneal for half an hour, then the walking traces were measured and the left sciatic nerves taken for pathological examinations, immunohistochemical staining NF-κB detections, ultrastructure observations, and the serums extracted for sero-enzyme LDH, MDA, SOD, GSH-Px measurements after 5 hours. The left iliac arteries of group I, group T were blocked with non-traumatic surgical artery clips for 3 hours, and the the clips were removed for blood stream for 2 hours. Then the left sciatic nerves of groupI, groupT were taken for pathological examinations, immuno