丹芪丸的临床前研究中药学专业论文.docxVIP

reasonable value. (2) Using TLC for astragalus membranaceus, salvia miltiorrhiza, epimedium, wine rhubarb, identify zedoary turmeric, thin layer map the spots were clear and the negative control showed no interference; Using HPLC determination Dan phenolic acid B content, in 0.7604～2.6614 μg range, Dan phenolic acid B peak area value and sample quantity has a good linear relationship, R is 0.9999. Average and sample recovery is 97.90%, RSD = 2.17% (n = 5), the quality control standard method is simple, reproducible and can effectively control the quality of the preparation. (3) We investigated three months of Danqi Wan listed packaging three batch samples, tested the indexes meet the quality standard and registered the pharmacopoeia of the Chinese Pharmacopoeia 2010 version of an appendix entry pill agent under the provisions of the provisional period. (4)Stilbene Danqi Wan to KM strain lavage did not measure LD50 in mice, 24 hours to 40ml/kg dose 3 times, observation for 14 days, mice without death；determine the maximum amount of drug is 66g/kg. Conclusion: Finished the new drug Danqi Wan preclinical studies. And the optimum extraction technology and molding process; established the quality control standards; Completed the preliminary stability test; urgent poison test shows that this product was safe. Key words Danqi Wan; preparation process; quality standards; stability; urgent poison Author: Li Ge Tutor: Yifan Feng 目 录 1 引言???????????????????????????????1 1.1 立题依据????????????????????????????1 1.2 可行性?????????????????????????????1 1.3 国内外研究概况?????????????????????????2 1.4 处方药物组成及方解???????????????????????2 1.5 临床使用背景??????????????????????????2 2 文献研究?????????????????????????????3 2.1 丹参??????????????????????????????3 2.2 黄芪??????????????????????????????4 2.3 淫羊藿?????????????????????????????6 2.4 酒大黄?????????????????????????????7 2.5