雌激素受体亚型在肥大乳房乳腺组织中的表达及意义-外科学(整形外科)专业论文.docxVIP

华 华 中 科 技 大 学 硕 士 学 位 论 文 PAGE 2 PAGE 2 Expression of estrogen receptor isoforms in the mammary gland of the hypertrophic breast and its significance Abstract Objective To detect the expression of the estrogen receptor(ER) isoforms(ERα and ERβ)in the mammary gland of the hypertrophic breast and the normal voluminal breast,so that their function and significance in the pathogenesis of breast hypertrophy can be investigated. Method The breast tissue samples were obtained,including hypertrophic breast tissue(38 cases) and normal voluminal breast tissue(17 cases).They were detected by the EnVision two step method immunohistochemistry for the expression of ERα and ERβ,and took statistical analysis to compare their difference.The pubertal hypertrophic breast and the postpartum hypertrophic breast were also compared. All the tissues were examined by pathology. Results ERα was expressed only in the cell nucleus mostly,and mainly in the breast epithelioglandulars.The positive expression rate of ERα in the hypertrophic breast was 97.37℅,while 76.47℅ in the normal voluminal breast,showing a significant difference between the two groups（P0.05）. The positive expression rates of ERα in the pubertal hypertrophic breast and the postpartum hypertrophic breast were 95℅and 100℅,and there was no significance between the two groups in a statistics basis （P0.05）. ERβ was expressed in both cytoplasm and nucleus,and it was expressed in many cell types,such as epithelioglandulars,stroma cells,myoepithelial cells and lymphocytes. The positive expression rates of ERβ in the hypertrophic breast and normal voluminal breast were 89.47℅ and 100℅,and showing no significant difference between them（P0.05）.So did the pubertal hypertrophic breast and the postpartum hypertrophic breast. Conclusions The development of hypertrophic breast may be related to the excessive expression of ERα in the breast tissue,while the relationship between the hypertrophic breast and ERβ may be not obvious，a