利用正常人溶血液作糖化血红蛋白质控物探讨.docVIP

利用正常人溶血液作糖化血红蛋白质控物探讨 　　【摘要】目的：用胶乳凝集法在全自动生化仪上测糖化血红蛋白(HbA1c)时，必需先将样品加溶血剂使红细胞彻底溶解,制成1∶40稀释液（简称溶血液 下同）再上机检测;本文探讨用溶血液作为胶乳凝集法测HbA1c的室内质控液的可行性。方法：选择符合条件的正常人全血，按试剂说明书载明的比例配制HbA1c测定用溶血液，后定值、分装和保存。从质控效果和稳定性对其进行评估。结果：自制正常人溶血液方法简便与购置的北京利德曼公司提供的定值质控品具相似的效果,当失控时能及时反映出来;4℃保存稳定期至少三月,-18℃保存稳定期半年。结论：可以用正常人溶血液代替胶乳凝集法糖化血红蛋白测定室内质控品。 　　【关键词】糖尿病; 糖化血红蛋白；胶乳凝集法；溶血液；室内质控 　　【Abstract】The purpose of using latex agglutination method in automatic biochemical analyzer measured glycosylated hemoglobin (HbA1c), the necessary increase in hemolytic agent first sample so that the total red blood cell lysis, made 1:40 dilution (referred to as dissolved with blood) to a aircraft detection; This paper discusses the use of blood as a soluble latex agglutination test the internal quality control HbA1c feasibility of liquid. Methods consistent with the conditions of normal whole blood, according to the proportion of reagent preparation manuals contain HbA1c determined by dissolving the blood, the latter value, packaging and preservation. Results from the quality control and stability of their assessment. The results of self-normal blood melting method is simple and the purchase of the Beijing Lidman value provided by quality control with similar results, when out of control can be reflected in a timely manner; 4 ℃ at least to preserve the stability of March, -18 ℃ to preserve the stability of period of six months. Conclusions can be used in place of normal blood soluble latex agglutination determination of glycosylated hemoglobin internal quality control materials. 　　【Key words】Diabetes;Glycosylated hemoglobin;Latex agglutination method;Dissolving blood;Internal quality control 　　【中图分类号】R446.11 【文献标识码】A 【文章编号】1006-1959（2009）06-0181-02 　　 　　HbAlc连续测定可以评价糖尿病控制水平;长期以来，评价糖尿病控制水平一直是个困难问题，对病情波动较大及注射胰岛素患者尤其如此。一次血糖、尿糖的测定只能反映抽血当时的血糖水平，况且血糖随进食和糖代谢的变化而有所改变，不能说明前一段较长时间病情的全貌，这给合理制定治疗方案带来了-定的困难，而HbAlc不随血糖变化而变化，可以反映出患者在抽血化验的前4～8周之内一段时间的血糖平均水平，正好可以弥补这一不足。近年来的研究表明：通过有规律地测量糖尿病患者治疗中HbAlc的浓度的下降，证实了HbAlc测定的临床意义[1]。因此，国外