川崎病动物模型的建立以与其血浆N末端脑利钠肽前体水平的变化.pdfVIP

安徽医科大学硕士学位论文 现其浓度相对于对照组有明显升高，差异具有统计学意义（P ﹤0.05），提示血浆 NT-proBNP 浓度变化可能是KD 血液中一种特异性变化的生化指标，有望成为临床 上帮助KD 早期诊断的指标之一。 关键词 川崎病 动物模型 N 末端脑钠肽前体 诊断 5 安徽医科大学硕士学位论文 Establishment of animal model of Kawasaki disease and the change of plasma N-terminal pro-brain natriuretic peptide levels Abstract Objective Kawasaki disease (KD) is a kind of common pediatric autoimmune disease, and it can cause multisystem vasculitis, which is leading to damage in the coronary artery and resulting in serious complications of coronary artery lesions(CAL). So, KD is becoming as the leading cause of acquired heart disease in children of the world ．Currently, KD is mainly based on clinical symptoms and ultrasound heartbeat graph to diagnose, lacking of specific biochemical markers for early diagnosis. This article summarizes some methods to establish an animal model of Kawasaki disease, and to measure the blood biochemical indexes, wondering to find a specific index and help the early diagnosis of the disease. While the pathogenesis of KD is unknown, the establishment of the animal model also provides the material basis for the further study . Methods Lactobacillus casei cell wall extract (LCWE) was used for immunization of genetically predisposed mice (BALB/C mice) to establish animal model of coronary arteritis. There were 40 mice as experimental group and 40 mice as control group. Mice （4-6 weeks old）were peritoneal injected with 0.5ml of NS containing 0.5mg LCWE or NS alone for 3 days, and then 8 mice in each group were sacrificed on days 1,3,5,10 and 28. Blood was collected, and cardiac tissues of mice were removed and preserved in formaldehyde. Then tissue specimens were stained with HE to observe the patho