低出生体重新生儿血清低分子量蛋白质谱图研究-临床医学专业论文.docx

的蛋白质峰有 148 个，质荷比分布为 2000～10000.MLBW 组与 NBW 组间 有差异（P0.05）的蛋白质峰有 84 个，质荷比分布为 2000～10000 范围.LBW 与 NBW 组间有显著统计学意义（P0.01）的蛋白质峰有 3 个，其蛋白表 达 量 依 次 是 2381.05 （ 3.05±2.17 ； 4.06±3.23 ）、 5057.57 （ 13.96±6.77 ； 8.20±4.74）、9444.82（13.28±4.33；9.79±4.87），其中 M／Z 为 2381.05 （3.05±2.17；4.06±3.23）在足月小样儿中组中低表达；而 M／Z 为 5057.57 （13.96±6.77；8.20±4.74）、9444.82（13.28±4.33；9.79±4.87）在低出生体 重早产儿组中高表达。并且在蛋白质数据库中搜索与上述 M／Z 相对应的 蛋白质。结论： 1、通过本次试验得出 LBW 有蛋白质代谢紊乱；2、LBW 可能由于体内蛋白质的表达缺损或不平衡造成某些蛋白含量较 NBW 低或 高。 关键词：低出生体重新生儿；小于胎龄儿；蛋白质组学；足月小样儿；表 面增强激光解吸/离子化飞行时间质谱；低出生体重早产儿；生物标志物； Research on serum low molecular weight protein Mass Spectrometry of Low birth weight infant Abstract： Objective: Low birth weight infants refers to the baby who,s birth weight less than 2500g. The general is premature or small for gestational age infants. The small for gestational age has another name is intrauterine growth retardation. Several studies have provided evidence that Low birth weight has a strong association between coronary artery disease, hypertension and stroke, type 2 diabetes, and osteoporosis in adulthood. Therefore, much more attention should be paid to the Low birth weight infant. we using protein chip combine with SELDI-TOF-MS to detect premature low birth weight infant, mature low bith weight infant and normal birth weight infant, Methods: including 37 cases of PLBW, 22 cases of MLBW,32 cases of NBW. Using Biomarker Wizard to analyze the data. Results: M/Z in range of 2000-10000, between the PLBW group and the control group, 148 protein peaks were statistically significant (p＜0.01), MLBW group and the control group,84 protein peaks were significant. There were 2381.05（3.05±2.17；4.06±3.23）、5057.57 （13.96±6.77；8.20±4.74）、9444.82（13.28±4.33；9.79±4.87），of these group including 2381.05（3.05±2.17；4.06±3.23）was lowly expressed in the MLBW group, including 5057.57 （ 13.96±6.77 ； 8.20±4.74 ）、 9444.82 （13.28±4.33；9.79±4.87）were highly expressed in the PLBW group. Conclusion: 1. In this