附子水溶性生物碱治疗急性心力衰竭的实验研究-药理学专业论文.docxVIP

成都中医药大学硕士学位论文 成都中医药大学硕士学位论文 万方数据 万方数据 溶性生物碱组细胞形态大多有所恢复，且丝状触角明显减少。附子水溶性生物碱 各剂量均有降低正常心肌细胞 Na+、Ca2+含量以及升高正常心肌细胞 K+含量的作 用，且各剂量组均能使 Ca2+-Mg2+-ATP 酶、Ca2+-ATP 酶与 Na+-K+-ATP 酶的活性 提高。经戊巴比妥钠造模后的心肌细胞，其膜上 Na+-K+-ATP 酶的活性增高，而 Ca2+-ATP 酶与 Ca2+-Mg2+-ATP 酶的活性出现降低，细胞内 Ca2+、K+的浓度升高， Na+与 Mg2+的浓度下降；阳性药与附子水溶性生物碱各剂量给药后，急性心力衰 竭模型细胞活力显著提高，且 Na+与 Mg2+的浓度与 Ca2+-ATP 酶、Ca2+-Mg2+-ATP 酶的活性均有所升高，Ca2+、K+的含量与 Na+-K+-ATP 酶的活性则有所降低，其 对急性心力衰竭细胞模型的作用趋势和强心苷类药物相仿。 结论：附子水溶性生物碱对急性心力衰竭大鼠模型以及急性心力衰竭细胞模 型都具有改善作用；能调节 HR、+dp/dtmax、 -dp/dtmax 使之趋于正常水平，并 可调节动物体内与急性心力衰竭相关的细胞因子；还能提高心肌细胞的活力、调 节心肌细胞内与急性心力衰竭相关的离子与 ATP 酶的含量，起到抗心力衰竭的 作用。 关键词：附子水溶性生物碱、急性心力衰竭模型、心肌细胞、离子、ATP 酶、 细胞因子 2 Abstract Objective: To studythe therapeutic effects of acontum fischeri mankshood water-solubility alkaloid on heart failure. Methods: ① Use propafenone hydrochloride to copy the model rats of AHF, After it, recording the HR and left ventricular pressure by BL-420 system. Then, Intravenous Inject acontum fischeri mankshood water-solubility alkaloid(0.025、0.050、 0.10ml/kg, the content of extractive:0.05g/ml) or positive drugSheng Mai （参麦） Injection （6.67g/kg), Measuring the change of HR、+dp/dtmax and -dp/dtmax after administration 5,10,20,30,60min.Adopt the same method to deal with anther batch of rats, anddetermine the concentration ofAng- Ⅰ ,Ang- Ⅱ ,TNF-α, ANP, BNP, ALD by blood sampling at the time point of 20min by ELISA.②Culture the myocardial cells, gotten from the neonatal rats, for 5 days until the cells becoming mature, then after usingNembutal (0.8%) to make the heart-failure cells model for 5min administrate them with 3 concentrations of acontum fischeri mankshood water-solubility alkaloid (0.01,0.02,0.04 g?L-1) and deslanoside injection (4mL ?L-1), observe the morphological characteristics of the cells and record the changes by taking photos 90min later.Culture the myocardial cells, administrate in the same way, determine the vitality of the myocardial cells’ membrane ATP enzyme and the concentration of relative io