灯盏细辛对大鼠肺缺血-再灌注损伤影响-普通外科学专业论文.docx

汕头大学医学院硕士学位论文 汕头大学医学院硕士学位论文 III III ABSTRACT Background and Objective Ischemia reperfusion injury of the lung (LIRI) is still a challenge for the thoracic surgeon, which occurs in many clinical conditions, such as cardiopulmonary bypass, lung embolism, shock etc, especially during the lung transplantation. Those may lead to acute lung injury or even multiple organ dysfunction syndrome (MODS). Ischemia–reperfusion induced lung injury contributes to a significant cause of morbidity and mortality after the lung transplantation, so it is necessary to better understand the mechanisms of lung ischemia reperfusion injury and investigate more reliable strategies to reduce the incidence of the injury. This experiment was carried out, through establishing an in situ hilar occlusion and in vivo rat lung ischemia reperfusion injury model. The wet-to-dry weight ratio (W/D), myeloperoxidase (MPO) of lung tissue and the content of nuclear NF-κB p65 were measured. The histopathological and ultrastructural changes of the lung tissue were observed, to explore the effects of erigeron breviscapine on ischemia reperfusion injury of rat lung in vivo. Materials and Methods Thirty-two male Sprague-Dawley rats were anesthetized with an intraperitoneal injection of sodium pentobarbital (30mg/kg). The animals were shaved, endotracheal intubated with a 14 gauge tube, and ventilated with a rodent ventilator at a respiratory rate of 60 breaths/min, a 1:1.5 of I/E ratio and a mean peak pressure of 0.02 MPa. All animals received 0.04 mg/kg of intramuscular atropine after being anesthetized. The chest was opened via a left thoracotomy through the fifth intercostal space. After the left pulmonary hilum including neural, vascular, lymphatic, and connective tissue was stripped, a ligature was placed through it, and the ends of the tie were threaded through a small plastic tube to form a snare for reversible left pulmonary hilar occlusion. Heparinization was maintained during the experimental period