镉铅胁迫下红树植物木榄生理生化特性、抗氧化酶和相关蛋白的研究-生物化学与分子生物学专业论文.docxVIP

PAGE PAGE IV A Study on physiological and biochemical properties, antioxidant enzymes and related proteins in a mangrove plant Bruguiera gymnorrhiza under cadmium and lead stress By: Tao Yi-ming Supervisor：Associate Professor Chen Yan-zhen Major：Biochemistry and molecular biology Specialty：Chemistry of enzyme and protein Grade: 2004 Abstract This study was carried out to investigate the effect of cadmium and lead on the growth, chlorophyll content, antioxidant enzymes and protein expression in a mangrove plant Bruguiera gymnorrhiza. The similarities and differences of Cd and Pb effects on B. gymnorrhiza are also discussed. The major resultes are summerized as follows: Under 20-30 mmol·L-1 Cd and 20-40 mmol·L-1 Pb treatment, B. gymnorrhiza seedlings grew slower than those treated with other concentrations and in control, with significant differences in plant height increasement. Concentrations of 30 mmol·L-1 Cd and 40 mmol·L-1 Pb led to wilt. Chlorophyll a, b, total chlorophyll and carotenoids experienced significant declines in all Cd and Pb treatments. The destruction of Pb to chlorophyll was stronger than Cd. The value of chlorophyll a/b increased suggesting that Cd and Pb exerted more destruction on chlorophyll b than on cholorophyll a in seedlings. Both Cd and Pb induced membrane lipid peroxidation in B. gymnorrhiza seedlings. The extent of lipid peroxidation caused by these two heavy metals was equivalent. The effects of Cd and Pb on POD and SOD activities in root and leaf extracts of B. gymnorrhiza seedlings were similar. The enzyme activites were stimulated in lower metal ion concentrations but were inhibited in higher concentrations. However, the effects of Cd and Pb on CAT activities in seedlings were quite different. The CAT activities were significantly inhibited when 1 mmol·L-1 Cd supplied suggesting the sensitivity of root CAT activities to Cd, while the enzyme activities experienced an initial increase and subsequent decrease when