地佐辛在雌激素干扰下对切口痛大鼠痛行为学及脊髓c-fos蛋白表达的影响麻醉学专业论文.docx

山西医科大学硕士学位论文 山西医科大学硕士学位论文 I I 地佐辛在雌激素干扰下对切口痛大鼠痛行 为学及脊髓 c-fos 蛋白表达的影响 摘要 目的：观察地佐辛在雌激素的干扰下对切口痛大鼠行为学及脊髓 c-fos 蛋白表达的影 响，探讨雌激素对地佐辛镇痛效果影响的可能机制。 方法：雌性SD大鼠32只，重量230-260g，摘除双侧卵巢饲养4周后，随机分为4组， 每组8只。分别为：对照组(C组)，雌激素组（E组），地佐辛组(D组)，雌激素+地佐辛组(E+D 组)。雌激素组：在造模前二小时经皮下注射17β-雌二醇0.2mg/100g；地佐辛组：在造模 后经尾静脉注射地佐辛0.1mg/100g；雌激素加地佐辛组：在造模前二个小时经皮下注射17 β-雌二醇0.2mg/100g；在造模后经尾静脉注射地佐辛0.1mg/100g；四组大鼠麻醉后,均用 Brennan法制作切口痛模型：对大鼠左后爪进行消毒，沿足底近端0.5cm处向趾部做一长约 1cm的切口，切开皮肤后，用眼科镊挑起足底肌肉并纵向切割，要保持肌肉起止及附着点 完整，按压止血后，用细线缝合皮肤两针。计数大鼠清醒后60min内12个时间段内的累积 疼痛评分。用ELISA法检测脊髓膨大处c-fos蛋白表达情况。 结果：同手术组(C组)比较，雌激素组（E组），地佐辛组(D组)，地佐辛+雌激素组(E+D 组)累计的疼痛评分均降低（P＜0.05），同时c-fos表达量也明显减少（P＜0.05）；同雌激 素组比较，地佐辛组以及地佐辛＋雌激素组累计疼痛评分以及c-fos表达均明显下降（P＜ 0.05）；同地佐辛组比较，地佐辛+雌激素组累计疼痛评分以及c-fos表达差异无统计学意 义（P＞0.05）； 结论：1. 地佐辛、雌激素，以及两者联合应用均有明显的镇痛作用，且均对 c-fos 表达有明显的抑制作用。 2.雌激素对地佐辛的镇痛效果无明显影响，两者联合应用镇痛效果没有增强。 关键词：地佐辛；雌激素；镇痛；原癌基因c-fos； Effect of Dezocine injection in estrogen interfering on pain behavior and spinal c-fos expression in rat models with incisional pain Objective: To investigate possible mechanism of dezocine injection in estrogen interfering and spinal c-fos expression in rat models with incisional pain. Methods:32 female SD rates were randomly assigned to 4 groups(n=8):removal ovary operation group(C) ;estrogen group （ E ） ;Dezocine group(D); estrogen and Dezocine group(E+D).Group of estrogen: 17beta-estradiol (0.2mg/100g)were injected subcutaneously two hours before modeling. Group of dezocine: After modeling, dezocine (0.1mg/100g) was injected via the tail vein. The combined group: 17beta-estradiol (0.2mg/100g) were injected subcutaneously two hours before modeling and dezocine (0.1mg/100g) was injected via the tail vein after modeling. After anesthetized, four groups of rats were making model of incisional pain with Brennan’s method. To disinfect the left hind paw of rats, making an incision(1cm) to toe along the proximal plantar 0.5cm.The plantar muscles were provoked and longitudinal cutted by ophthalmic tweezers. To keep the mus