补充赖氨酸对大鼠心肝组织Ｂａｘ和Ｂｃｌ－２影响.docVIP

补充赖氨酸对大鼠心肝组织Ｂａｘ和Ｂｃｌ－２影响 　　摘要：为了解补充不同浓度赖氨酸对力竭运动大鼠心、肝组织细胞凋亡的影响，观察其对心、肝组织细胞凋亡调控基因Bax和Bcl-2的变化，探讨赖氨酸对力竭运动大鼠机体的保护作用。将32只大鼠进行3 d的跑台运动适应性训练后，随机分成4组：安静对照组、运动组、低浓度给药组和高浓度给药组。力竭运动大鼠均采用Bedford所建立的运动负荷模型。实验结束用免疫组织化学法检测心、肝组织细胞凋亡调控基因Bax和Bcl-2的阳性物质平均光密度值、面积和含量。结果：与对照组比较，运动组和给药组心、肝组织细胞凋亡调控基因Bax光密度值差异具有显著性意义(P?0.05)，与运动组比较，差异也具有显著性(P?0.05)，不同浓度赖氨酸的作用相当，差异没有显著性；运动组与对照组比较，心、肝组织细胞凋亡调控基因Bcl-2光密度值差异具有显著性意义(P?0.05)，给药组与运动组比较，心组织细胞凋亡调控基因Bcl-2光密度值差异也具有显著性意义(P?0.05)，只肝细胞表现高浓度给药组比低浓度给药组相比差异有显著性。结果说明：大强度急性力竭运动可使大鼠心、肝组织致损伤，诱导肝细胞凋亡，L-赖氨酸对急性力竭运动的大鼠心、肝组织有保护作用。 　　关键词：大强度急性力竭运动；L-赖氨酸；细胞凋亡调控基因；免疫组织化学 　　中图分类号：G804.7文献标识码：A文章编号：1006-7116(2008)05-0094-05 　　 　　Effects of lysine supplement on Bax and Bcl-2 of cardiac and hepatic tissue of rats 　　LUO Rong-bao1，ZHANG Gui-lan2，LIU Wen-feng3，LIU Hui-min3 　　（1.Department of Physical Education，Xiangnan College，Chenzhou 423000，China；2.Department of 　　Physical Education，Changsha Institute of Vocational and Technical Aviation，Changsha 400124，China； 　　3.School of Physical Education，Hunan Normal University，Changsha 410012，China） 　　 　　Abstract: In order to gain an insight into the effects of lysine with different concentrations on the apoptosis of cardiac and hepatic histiocytes of rats doing an exhaustive exercise, to observe the change which lysine causes to Bax and Bcl-2 (genes that regulate the apoptosis of cardiac and hepatic histiocytes), and to probe into the function of lysine in protecting the body of rats doing an exhaustive exercise, the authors divided 32 rats randomly into 4 groups (calm control group, exercise group, low concentration lysine supplied group and high concentration lysine supplied group) for 3 days of adaptation training on a running track, adopted the exercise load model established by Bedford for rats doing an exhaustive exercise, used an immunohistochemical method to test the average optical density, area and content of the positive substance of Bax and Bcl-2 when the experiment was finished, and revealed the following findi