化脓性脑膜炎脑脊液致病菌高通量测序及物种分析-神经病学专业论文.docxVIP

宁夏医科大学硕士学位论文 摘要  PAGE 5 的种类，丰度及菌群结构的分析发现银川地区感染的优势菌群为：链球菌，鲍曼不动杆 菌及假单胞菌，还有一些未知的细菌也是造成颅内感染的重要元凶。 关键词：化脓性脑膜炎，脑脊液，细菌培养，16S rDNA PCR 扩增，16S rDNA 宏 基因组测序 宁夏医科大学硕士学位论文 英文摘要 Pathogenic Bacteria High-Throughput Sequencing and Species analysis in Cerebrospinal Fluid with Purulent Meningitis ABSTRACT Objective Research in common types of bacteria，abundance of bacterial and structure of community microbial in the cerebrospinal fluid of patients with purulent meningitis pathogens，and to explore a new way of the etiological diagnosis with purulent meningitis. Methods Select 61 cerebrospinal fluid samples of the clinical diagnosis purulent meningitis patients in this study. Extract bacterial genomic DNA of the purulent meningitis patients in CSF and then identified by PCR. Culture the bacterial of CSF. Calculate the positive rate of these two methods，and compared with the two positive rate by the chi-square test with spss17.0 statistical software. Use high-throughput sequencing for the PCR-positive samples by 16S rDNA metagenomic sequencing technology，and then integrate and analysis the sequencing results by bioinformatics analysis method. Conclusions Culture and test bacterial for 61 CSF samples with purulent meningitis patients，there are 26 specimens amplified specific DNA product at 550bp，and bacterial culture specimens are only 10 cases. The positive rate of 16S rDNA PCR is 42.6%. The positive rate of bacterial culture is 16.4%. The results of 16S rDNA metagenomic sequencing technology and bioinformatics techniques: CSF containing the species is relatively less than other environment by the observing the OTU number of each sample. The type of bacteria can be roughly divided into Firmicutes and Proteus at the phylum in the purulent meningitis CSF samples. The main species of bacteria are Streptococcus, Acinetobacter, Pseudomonas and Neisseria at the genus. Analysis by beta diversity, the  bacterial species of purulent meningitis CSF are little differences between a