胃癌中HER-2／neu基因扩增和蛋白表达的多中心研究.docVIP

胃癌中HER-2／neu基因扩增和蛋白表达的多中心研究 中华消化杂志2006年10月第26雀笙塑』!!!!:!: 胃癌中HER一2/neu基因扩增和蛋白表达的 多中心研究 HER一2研究全国协作组 【摘要】目的观察中国人胃癌HER一2/neu基因扩增和蛋白表达状况,探讨两者间关系及与患者 临床病理参数间的关系.方法前瞻性研究中挑选胃腺癌蜡块270例,回顾性研究中挑选蜡块277例, 分别用显色原位杂交(CISH)和免疫组化法(IHC)检测HER一2/neu基因扩增和蛋白表达状况.结果 胃癌前瞻性病例中HER-2/neu基因扩增率为14.8%,蛋白过表达率为11.9.肠型胃癌HER-2/neu 基因扩增率和蛋白过表达率分别为25.4和18.8%,明显高于弥漫型胃癌的4.7和5.5(P值均lt; 0.01).高,中度分化胃癌HER一2/neu基因扩增率和蛋白过表达率均明显高于低分化胃癌(P值均lt; 0.01).回顾性,前瞻性研究及汇总后的总结果中HER一2/neu蛋白CISH和IHC检测结果符合率分别为 77.0,89.2,83.2,两者显着相关(P值均lt;0.01).结论HER一2/neu基因扩增,蛋白过表达可 能与中国人胃癌发生有关,基因扩增可能是其蛋白过表达的主要分子机制,HER_2/neu基因扩增和蛋白 过表达与肠型胃癌和高中度分化胃癌相关. 【关键词】胃癌;基因扩增;蛋白表达 MulticenterstudyonHER-2/neugeneamplificationandproteinexpressioninpatientswithgastriccancer CooperativeStudyGroupofHER一2inChina Correspondingauthor:ZHANGGui—hong,PathologyDepartment,CancerHospitalAffiliatedto FudanUniversity,Shanghai200032,China [Abstract]ObjectiveToinvestigatetheamplificationandproteinexpressionofHER一2/neugene, andtheirrelationshipwithclinicalpathologicalparameters.Therelationshipbetweenamplificationwith proteinexpressionwasalsostudied.MethodsTwohundredsandseventysamples(duringMaytoSep— tember2005)and277samples(duringFebruary2001toMay2005)werecollectedfrompatientswith gastricadenocarcinomainprospectivestudyandretrospectivestudy,respectively.HER一2/neugeneam— plificationwasmeasuredbychromogenicinsituhybridization(CISH)andproteinexpressionwasdetec— tedbyimmunohistochemistry(IHC).Results(1)Inprospectivestudy,theamplificationandprotein overexpressionofHER一2/neugenewere14.8and11.9respectively.Buttheywere25.4and 18.8inintestinaltypegastriccarcinomas,whichweresignificantlyhigherthanthoseindiffusetype gastriccarcinomas(4.7and5.5,Plt;0.01).ThepercentageofHER一2/neugeneamplificationand proteinoverexpressioninwellandmoderately—differentiatedgastriccarcinomasweresignificantlyhigher thanthoseinpoorlydifferentiatedones.(2)ConcordantpercentagebetweenIHCandCISHwas77.0, 89.2and83.2,inretrospective,prospectivestudiesandtotalresults,respectively.Tworesults havesignificantcorrela