河南道地药用植物辛夷内生真菌Y111的初步研究-生物化学与分子生物学专业论文.docxVIP

摘 要 4．通过对辛夷内生真菌Y111挥发油化学成分的气相色谱-质谱联用分析，发 现了辛夷内生真菌Y111与其宿主植物辛夷含有相同的化学成分，他们分别是石 竹烯、E,E-金合欢醛、桉树脑等，并且这些成分大都是辛夷植物中的主要成分。 关 键 词：内生真菌，辛夷，发酵条件，抗菌，菌种鉴定，挥发油成分 论文类型：应用基础研究 II 摘 要 Subject: Primary studies on endophytic fungi Y111 in Lily Magnolia from HeNan genuine medicinal plant Specialty: Biochemistry and Molecular Biology Name: Liu Xiaojie Supervisor: Prof. He Jia ABSTRACT Endophytic fungi are the fungi associated with living tissue of plants without causing any noticeable symptoms of disease. Recent years, studies on entophytic fungi showed that some antibacterial and antitumor products made by them. It is consent that endophytic fungui are new microbes resource which has latent value for exploitation. At the present stage, our study on medicinal plant endophytic fungi is just beginning. We can not take full advantages of entophytic fungi because many of their characters have not been understood. So study on entophytic fungi in medicinal plant not only can bring great social benefits, but also have the realistic theoretical significance. In this scientific dissertation, studies on the object that endophytic fungui Y111 in medicinal plant Lily Magnolia. The results obtained as following: According to the growth rates of mycelia on different media, an optimum solid medium suitable strain Y111 was PDA. Through analysing the amount of biomass and the effect of inhibit bacteria on different liquid media, an optimum liquid medium suitable Y111 was selected out then, it was PDA. On the basis medium of PDA, the fermentation media of strain Y111 were further optimized by the single factor and orthogonal experiment. The results showed that 20% of the potato juice by adding 4% glucose, 0.3% peptone, 0.1% K2HPO4 were the optimum conditions for its mycelial growth, 20% of the potato juice by adding 4% glucose, 0.3% peptone, 0.2% K2HPO4 were suitable for inhibit bacteria. With antibacterial activity as an indicator, using the single factor and orthogonal experiment optimize