高三尖杉酯碱抑制兔LASEK术后角膜haze形成的实验研究-眼科专业论文.docxVIP

PAGE PAGE 10 短时贴覆给药方式及HHT低浓度术后点药方式均能显著抑制haze的形成。本研究 为将来HHT应用于临床防治LASEK术后角膜haze提供实验依据及理论基础。 关键词 高三尖杉酯碱；准分子激光上皮下角膜磨镶术；角膜上皮下雾状混浊 Experimental study on homoharringtonine inhibiting haze after laser subepithelial keratomileusis in rabbits ABSTRACT Objective To investigate the therapeutic effect of Homoharrin- gtonine (HHT) on corneal haze formation after laser subepithelial keratomileusis (LASEK) in rabbits. Methods This study was a prospective ， randomized animal experiment. Forty-eight New Zealand white rabbits were underwent -10.0 diopters laser subepithelial keratomileusis(LASEK) . All eyes were randomLy divided into 4 groups by self-control ， group A：1mg/mL HHT during operation treating group ，group B： 0.1mg/mL HHT after operation treating group，group C：0.1% fluorometholone (FML) after operation treating group，group D： balanced salt solution (BBS) after operation treating group as control group. And there were twenty-four eyes in each group . PartⅠ：After the operation ， corneal epithelium healing time , ocular objective sign and corneal haze grades were periodicly observed and evaluated by slit lamp microscope . PartⅡ： Histopathologic examinations were made with light microscope when1,2week,1,2,3,and 6 month after the operation ,and with transmission electron microscope when 1,3,and 6 month after the operation . Results The time of reepithelialization were insignificant differences among all groups (P＞0 05). Corneal haze grades after surgery were significant differences among all groups (P＜0.05),and were significant differences at different observation time point postoperatively (P＜0.05), and the role of time factor do not differ with the difference of group(P＞0.05). Pairwise comparisons at different observation time point of outcomes showed that at 1 month and 2 months after the operation ,the Corneal haze grades was significantly less in group A, group