Apn MDHIGFI基因对京海黄鸡重要经济性状的遗传效应研究.docx

优秀毕业论文 精品参考文献资料 刘大林 刘大林 Apn、MDH和IGF．I基因对京海黄鸡重要经济性状的遗传效应研究 巴 Research on Genetic Effect of Apn Gene，MDH Gene and IGF··I Gene on Economical Traits in Jinghai Yellow Chicken AB STRACT PCR-SSCP and PCR-RFLP method was used to study the SNP of Apn (Adiponectin)Gene，MDH(Malate Dehydrogenase)Gene and IGF-I(Insulin—like growth factor-I)Gene that might be correlated with fat deposit traits in Jinghai Yellow chicken，and the systematic investigation was conducted on Jinghai Yellow chicken Apn Gene，MD HGene and IGF-I genes to evaluate the association of molecular markers 谢th growth traits，reproduction traits，CarCasS traits and meat quality traits． The main results are listed as follows： 1．Parts of 5-flanking rein and whole code region DNA of Apn gene were obtained using the techniques of PCR·SSCE 7 Apn SNPs were found in Jinghai Yelow chicken：C1251G，C1608T，G1773A，A1782G，T1902C，G1993C and G2013A，and they were all synonymous SNP．A single mutation at the position of the 235 bp of MD 5-flanking region Was found．A SNP of lGF—I Was detected． 2．AD2、AD7、MD and P1 1 locus have medium level PIC value，AD5 and AD6 have high level PIC value． 3．At the AD2 locus of Apn gene，birds with AA genotype has significant lowei value of age at first egg than the birds with AB genotype(P0．05)，birds with AA genotype has significant higher value of egg numbers at 300 days than the birds、析th AB genotype(P0．05)． At the AD5 locus of Apn gene，birds、析tIl h3h5 genotype has significant lower value of age at first egg than the birds with hl hl genotype(P0．05)，birds丽m h3h5 genotype has significant higher value of egg numbers at 300 days and 66 weeks than the birds 盟 盟 扬州大学博士学位论文 with hlhl genotype(P0．05)．At the AD6 locus of Apn gene，h2h5 genotype was the predominant genotype that increased egg numbers． At the AD7 locus of Apn gene．birds、析tll GG genotype has significant lower value of age at first egg than the birds with AA genotype(P0．05)，birds“tll GG genotype