高迁移率族蛋白B1在肝纤维化发生发展中的作用及其机制的研究-内科学(传染病)专业论文.docxVIP

山西医科大学硕士学位论文 山西医科大学硕士学位论文 IV IV Results: HMGB1 could activate HSC significantly: The levels of α-SMA, TGF-β in HSCs without stimulation were very low by immunohistochemistry, however, the expression of them increased obviously after been stimulated by HMGB1(P＜0.05). Pretreated with anti-HMGB1, the contents of α-SMA and TGF-β1 reduced markedly compared with HMGB1 group (P＜0.05), but the contents of them have no significant difference compared with control group(P＞0.05). The levels of HA, PIIIP, CIV and TGF-β1 of contral group was very low in serum measured by ELISA, however, The levels of them in HMGB1 group were much higher than in contral group(P＜0.05). Pretreated with anti-HMGB1, the levels of HA, PIIIP, PIVP and TGF-β1 reduced significantly compared with HMGB1 group (P＜0.05). but the levels of them have no significant difference compared with control group(P＞0.05). RAGE antibody could inhibit the activation of HMGB1 to HSC: In Anti-RAGE+HMGBl group, the levels of α-SMA, TGF-β1 in HSC was lower than HMGB1 group by immunohistochemistry(P＜0.05). The levels of HA, PIIIP, CIV and TGF-β1 in serum of contral group was very low compared with HMGB1 group by ELISA, however, The levels of them in HMGB1 group were much higher than in contral group(P＜0.05). In Anti-RAGE+HMGBl group, the expression of α-SMA, TGF-β1 by immunohistochemistry and The levels of HA, PIIIP, CIV TGF-β1 by ELISA were increased relatively compared with control group, but the differences was not statistically significant (P＞0.05). sRAGE could antagonist the activation of HMGB1 to HSC: In sRAGE+HMGBl group, the levels of α-SMA, TGF-β1 in HSC decreased apparently compared with HMGB1 group by immunohistochemistry(P＜0.05). the content of HA, PIIIP, PIVP and TGF-β1 in supernatant fluid decreased markedly compared with HMGB1 group(P＜0.05). the expression of α-SMA, TGF-β1 by immunohistochemistry and the levels of HA, PIIIP, CIV TGF-β1 by ELISA were increased relatively compared with co