原发性IgA肾病中微小RNA的差异表达研究.docVIP

原发性IgA肾病中微小RNA的差异表达研究 眭维国，蓝慧娟，晏 强，邹贵勉，李 飞，郑艺花，黄 河，陈洁晶，戴 勇 （中国人民解放军第181医院肾脏科，广西 桂林541002） 摘 要：目的 借助微小RNA（microRNA，miRNA）芯片研究IgA肾病（IgA nephropathy，IgAN）中miRNA差异表达。方法 收集11例IgAN患者肾穿标本作为IgAN组，3例镜下病理检查正常的肾皮质作为对照组。使用Trizol提取每组总RNA,分离miRNA，采用miRNA芯片筛选出差异表达显著的miRNA，实时定量PCR技术验证芯片结果可靠性。结果 IgAN中miRNA出现显著差异表达65个，其中显著表达上调34个，显著表达下调31个。挑选hsa-miR-637、hsa-miR-492进行实时定量PCR相对定量检测，结果显示2种方法检测的miRNA表达量和表达趋势相符，芯片结果具有可靠性。结论 IgAN是一种经典的多因素、多基因病，目前其机制尚未明确。本实验表明miRNA在IgAN中出现显著差异表达，深入研究将有助于IgAN机理研究及其诊断、治疗和预防。 关键词：微小RNA表达；微小RNA芯片；IgA肾病 中图法分类号： 文献标识码： The studies of MicroRNAs differential expression in IgA nephropathy Weiguo Sui, Huijuan Lan, Qiang Yan,Guimian Zou,Fei Li,Yihua Zheng,He Huang,Jiejing Chen,Yong Dai （Kidney Transplantation and Hemo Purification Center, 181 Hospital of Guilin, Guangxi Province, 541000, China） Abstract：Objective Studied microRNAs differential expression in IgA nephropathy using microRNAs array．Methods Collected biopsies of 11 patients with IgA nephropathy as IgA nephropathy group and 3 renal cortexes of tissue eumorphism patients as normal control group. Total RNA of every group was extracted using Trizol according to the manufacturer’s instruction. MicroRNAs were isolated from total RNA and significant differential expression microRNAs were screened by microRNAs array.The reliability of the results of microRNAs array was validated by Realtime PCR. Results In IgA nephropathy, there were 65 microRNAs significant differential expression of which 34 up-regulated and 31 down-regulated. Hsa-miR-637，hsa-miR-492 were selected for Relative quantification by Realtime PCR. It showed that the results of both methods were not significant deviation and the results of microRNAs array was reliable.Conclusion IgA nephropathy was a classic multiple factors, polygenic disease and until now there was no mechanism which can identify it perfectly. Our data indicated that microRNAs were significant differential expression in IgA nephropathy. The lucubrated of micr