骨形态发生蛋白2联合热化疗诱导人结肠癌细胞的增殖抑制作用及机制.docVIP

骨形态发生蛋白2联合热化疗诱导人结肠癌细胞的增殖抑制作用及机制 龙腾 王亚旭 作者单位：400010，重庆医科大学附属第二医院普外科 通信作者：王亚旭，Email:wangyaxu1967@ 【摘要】目的 探讨骨形态发生蛋白-2（BMP-2）、热化疗(五氟尿嘧啶，5-FU)单独和联合应用对人结肠癌细胞（SW480）株增殖凋亡的影响并探讨其可能机制。方法 BMP-2（100μg/L）、5-FU（75mg/L、100mg/L）单独与联合作用于SW480细胞株，FCM法检测细胞凋亡率，Western blot法检测PTEN(人第10号染色体缺失的磷酸酶及张力蛋白同源的基因)蛋白的表达，逆转录聚合酶链式反映（RT-PCR）检测PTEN mRNA表达。结果 与单独作用比较，BMP-2联合热化疗(5-FU)作用于SW480细胞株时，凋亡率明显增高（P0.01)。BMP-2（100μg/L）与5-FU(75mg/L)联合使用与5-FU（100mg/L）单独使用相比，其细胞凋亡率无明显差别（P0.05)。BMP-2联合热化疗(5-FU)作用时PTEN蛋白的表达水平高于单独使用热化疗（5-FU）时的表达水平。各组间PTENmRNA的表达没有差异。 结论 BMP-2与热化疗（5-FU）联合作用有协同诱导SW480细胞株凋亡的作用，并可以降低化疗药物剂量，其机制可能与上调PTEN蛋白表达有关。 【关键词】 热化疗；骨形态发生蛋白2；PTEN；细胞凋亡 The effect and mechanism of bone morphogenetic protein-2 on cell proliferation inhibition by combining thermo-chemotherapy in colon cancer SW480 cells Author：Long Teng Mailing address: General surgery of Chongqing Medical university affiliated second hospital; Chongqing 400010，China Corresponding author: Wang Yaxu 【Key word】Thermo-chemotherapy; bone morphogenetic protein-2; PTEN; apoptosis [Abstract] Objective To observe the effects bone morphogenetic protein-2 (BMP-2)、Thermo-chemotherapy (5-FU) and their combination on the proliferation and apoptosis of human colon cancer cell（SW480） line, and to explore the possible mechanism. Methods BMP-2(100μg/L) and 5-FU(75mg/L、100mg/L) were used, SW480 cells were divided into control group, BMP-2 treated group, Thermo-chemotherapy (5-FU) treated group, Thermo-chemotherapy(5-FU)+BMP-2 treated group. Their apoptosis rate was detected by flow cytometry (FCM). The protein expression of phosphatase and tensin homolog deleted on chromosome ten (PTEN) was detected by Western blotting. The mRNA expression of PTEN was detected by reverse transcription PCR （RT-PCR）. Results The apoptosis rate of Thermo-chemotherapy (5-FU)+BMP-2 treated cells was significantly higher than those of BMP-2 treated and those of Thermo-chemotherapy(5-FU) treated cells（P＜0.01 ). The apoptosis rate of SW480 cells treated with BMP-2 (100μg/