霉酚酸酯对小鼠肺纤维化的治疗作用.docVIP

霉酚酸酯对小鼠肺纤维化的治疗作用 曹 姝，兰 曦，王 勇，邹冬玲，臧春宝，王 颖?[基金项目] [通信作者]：王 颖，电话：（023E-mail: ?[基金项目] [通信作者]：王 颖，电话：（023E-mail: wangying_cq@ [摘要] 目的 初步探讨霉酚酸酯（mycophenolate mofetil,MMF）对博莱霉素（bleomycin,BLM）诱导的小鼠肺纤维化模型的治疗作用。方法 将36只C57BL/6小鼠随机具体是什么随机分组方法？分为6组：正常对照组、MMF对照组、BLM模型组及MMF干预组（分为低、中、高剂量组），每组6只。实验第1天BLM模型组、MMF干预组经气管注入BLM（6mg/kg），从实验第2天开始MMF干预组分别给予低、中、高剂量MMF(20、60、100 mg/kg)正常对照组、MMF对照组怎么处理的？剂量多大？，2周后处死所有小鼠，收集肺脏标本；HE、Masson染色在组织形态学上观察肺纤维化病变情况，Aschcroft法评分；RT-PCR检测COLA1及COLA2的mRNA表达水平；免疫组化法检测肺组织TGF-β1的蛋白表达。结果 BLM诱导的肺纤维化模型构建成功；MMF高剂量干预组与BLM模型组比较，前者的纤维化程度减轻，COLA1、COLA2 mRNA表达及TGF-β1蛋白表达明显降低（P0.01）。而低、中剂量组与BLM模型组间、MMF对照组与正常对照组间差异无统计学意义(P0.05)。结论 MMF能有效减轻BLM诱导的肺纤维化病变，其机制可能与下调促纤维化细胞因子TGF-β1相关。 具体是什么随机分组方法？ 正常对照组、MMF对照组怎么处理的？剂量多大？ [关键词] 霉酚酸酯；博莱霉素；肺纤维化；转化生长因子-β1；Ⅰ型胶原 [中图法分类号] [文献标志志码] A Mechanism of mycophenolate mofetil on treating pulmonary fibrosis mice models Cao Shu, Lan Xi, Wang Yong, Zou Dongling, Zang Chunbao, Wang Ying(Department of Radiology, Chongqing Medical University, Chongqing, 400016 [Abstract] Objective To probe into the mechanism of mycophenolate mofetil(MMF) in treating bleomycin(BLM)-induced pulmonary fibrosis of mice. Methods 36 mice were randomly divided into 6 groups(6 mice in each group), including normal control group, MMF control group, BLM model group and MMF treatment groups which were consist of low, media and high dose. One day after intratracheally instilled BLM(6mg/kg) which was dissolved in saline in BLM model group and MMF treatment groups, MMF was given orally in MMF treatment groups(20,60,100 mg/kg) once daily for two weeks. All mice were killed two weeks later, and lung tissues were collected for the following experiments. Pulmonary fibrosis was evaluated by Ashcroft score in HE and masson trichrome stain. The expression of COLA1 and COLA2 mRNA were determined by RT-PCR. TGF-β1 protein were analyzed by immunohistochemistry. Results BLM-induced pulmonary fibrosis model was successfully construct