老年大鼠胼胝体额区有髓神经纤维与髓鞘改变的研究.docVIP

老年大鼠胼胝体额区有髓神经纤维与髓鞘改变的研究? ?基金项目：国家自然科学基金；国家自然科学基金；国家人事部2005年度高层次留学人才回国工作资助经费；教育部留学回国人员科研启动基金；教育部重点项目；重庆市自然科学基金（CSTC,2005BB5034）重庆市教委基金项目 作者简介：徐瑲（1978-），男，汉族，陕西西安人，泸州医学院组织胚胎学教研室 ＊通讯作者：唐勇 教授 博士生导师Email:ytang062@ (023 徐瑲1 卢伟2,3 唐勇2,3＊ （1泸州医学院组织胚胎学教研室， 泸州 646000；2 重庆医科大学组织胚胎学教研室，3重庆医科大学干细胞与组织工程学研究室，重庆 400016） 摘要 目的 探讨老年大鼠胼胝体额区有髓神经纤维、有髓神经纤维髓鞘和髓鞘碱性蛋白（MBP）变化。方法 运用透射电子显微镜和新的体视学方法分别对年轻组和老年组雌性Long-Evans大鼠胼胝体额区有髓神经纤维进行定量研究， 运用Western-blot对年轻组和老年组大鼠胼胝体额区MBP进行定量研究。结果 与年轻组比较，老年组胼胝体额区体积存在显著性降低，胼胝体额区有髓神经纤维总体积显著性下降，Mr 21.5×104 MBP表达量显著性降低。Mr 21.5×104 MBP与有髓神经纤维总体积、髓鞘总体积间呈正相关。结论 胼胝体存在显著性老年萎缩，胼胝体额区有髓神经纤维的老年改变可能与老年大脑额叶功能下降有关。 关键词 胼胝体额区 髓鞘碱性蛋白 有髓神经纤维 体视学 老年改变 MYELIN IN THE FRONTAL REGION OF CORPUS CALLOSUM IN OLD RATS XU Qiang1 LU Wei2,3 TANG Yong2,3△ (1. Department of Histology and Embryology, Luzhou Medical College, Luzhou 646000; 2. Department of Histology and Embryology, 3.Laboratory of stem cell and tissue engineering, Chongqing Medical Abstract Objective To explore the age-related changes of the myelinated fibers, myelin sheath and myelin basic protein in the frontal region of the corpus callosum in old rats. Methods The myelinated fibers in the frontal region of corpus callosum of young and old-aged female Long-Evans rats were quantitatively studied using electron microscope and unbiased stereological techniques. Myelin basic protein in the frontal region of corpus callosum of young and old-aged female rats were quantitatively studied using Western-blot. Results When compared to young female rats, the total volume of the frontal region of the corpus callosum and the total volume of the myelinated fibers in the frontal region of old corpus callosum were significantly degraded. The expression of Mr 21.5×104 MBP was significantly decreased in old rats. The expression of Mr 21.5×104 MBP, the total volume of the myelinated fibers and the total volume of the myelin sheathes were positisely correlated. Conclusion There was age-rela