胸腺肽α1体外刺激胃癌患者PBMCs对淋巴细胞亚群影响.docVIP

胸腺肽α1 体外刺激胃癌患者PBMCs对淋巴细胞亚群影响 杨 霞1* ，钱 锋2* ，刘凯军1 ，何海洋1，兰远志2， 田 易1 ，傅晓岚1 ，李 健1 ，张 记1 ，申子刚1，李晋涛1，吴玉章1（400038重庆，第三军医大学：全军免疫研究所1；西南医院普通外科2） [摘要] 目的 观察胸腺肽α1（Tα1） 体外刺激培养胃癌患者外周血单个核细胞（peripheral blood mononuclear cells， PBMCs）对淋巴细胞亚群分化及分泌细胞因子谱影响，分析Tα1对肿瘤患者免疫功能的影响。方法 分离32例胃癌患者及18例健康自愿者PBMCs，Tα1 50、10及1 μg/ml体外刺激培养2 d，流式细胞仪检测CD4+、CD8+、CD4+ CD25+ Foxp3+ T细胞百分比，CD4+/CD8+变化，及分泌Th1/Th2细胞因子谱变化。结果 Tα1体外刺激PBMCs后， 胃癌患者及健康自愿者的CD4+、 CD8+ T细胞水平及CD4+/CD8+比值均无明显变化；健康自愿者CD4+ CD25+ Foxp3+ T细胞水平无明显变化，胃癌患者CD4+ CD25+ Foxp3+ T细胞水平明显提高 (P＜0.05)；Tα11 μg/ml促进胃癌患者PBMCs分泌IL-1β、TNF-α，10 μg/ml促进胃癌患者PBMCs分泌IL-6，健康人PBMCs分泌细胞因子谱变化不明显。结论：Tα1体外刺激胃癌患者PBMCs，提高CD4+ CD25+ Foxp3+ Treg细胞亚群水平，可能对肿瘤患者抗肿瘤免疫起抑制效应。 [关键词]胸腺肽α1（Tα1）；外周血单个核细胞（PBMC）；Foxp3+；Th1/Th2细胞因子谱 [中图法分类号] [文献标识码] A Effect of thymosin α1 on the percentage of T-lymphocyte subsets in patients with gastric cancer in vitro Yang Xia1*，Qian Feng2*，Liu Kaijun1，He Haiyang1， Lan Yuanzhi2，Tian Yi1，Fu Xiaolan1，Li Jian1，Zhang Ji1，Shen Zigang1，Li Jintao 1 ，Wu Yuzhang1 (1Institute of Immunology and 2Department of General Surgery， Southwest Hospital， Third Military Medical University， Chongqing， 400038， China ) 【Objective】 To investigate the effect of thymosin α1 on cellular immune function in patients with gastric cancer. Methods： PBMCs were obtained by centrifugation of blood from 18 healthy subjects and 32 patients with gastric cancer over ficoll solution， then cultured in the presence of culture medium with addition of Tα1 at 50、10 and 1μg/ml for 2 days. T lymphocyte subsets (such as CD4+， CD8+ and CD4+ CD25+ Foxp3+ T cells) and Th1/Th2 multiplex cytokines were detected by flow cytometry (FCM). Results： in vitro studies， PBMCs isolated from healthy people and patients incubation with or without Tα1， and there was no significant change in percentage of CD4+， CD8+ peripheral lymphocyte subsets and ratio of CD4+/CD8+. But there had a statistically significant upgrade of CD4+ CD25+ Foxp3+ T lymphocyte subsets from patients