化合物脂水分配系数的毛细管电泳峰漂移法研究化学专业论文.docxVIP

重庆大学硕士学位论文英文 重庆大学硕士学位论文 英文摘要 PAGE PAGE IV ABSTRACT In conventional microemulsion electrokinetic chromatography (MEEKC), a series of reference compounds and the migration time of microemulsion phase are required to determine the octanol-water partition coefficient (logPo/w) of compounds. The accuracy of the obtained logPo/w is depended on the correctness of the logPo/w of reference compounds and the migration time of microemulsion phase. Thus, a novel MEEKC method based on peak-shift assay (p-s MEEKC) was developed to measure logPo/w value of compounds. Here, a series of microemulsion electrolytes with gradient content of microemulsion droplets were employed. And the logPo/w value of compounds could be calculated according to the changes in effective mobility of analyte as a function of the amount of microemulsion droplets in the electrophoresis electrolytes. Based on this, the logPo/w value of compounds and drugs were studied. Then, the use of microemulsion composed of nonionic surfactant to determine the logPo/w value of charged compounds was proposed. Moreover, relative mobility substitited for mobility was developed to describe the migration of analytes and used to estimate the hydrophobicity of drugs. In this thesis, the main research work and obtained results are as follows: ① According to the changes in effective mobility of analyte as a function of the amount of microemulsion droplets, a novel MEEKC method based on peak-shift assay (p-s MEEKC) was developed. Then, the relationship between the concentration of analytes and effective mobility was investigated. Furthermore, the logPo/w of p-nitroaniline was studied to prove the reliability of the method. ② The logPo/w values of 11 compounds were determined using the proposed p-s MEEKC and conventional MEEKC method. Then, the determined values by the two methods were compared with reference value. It is showed that the logPo/w values obtained by the developed p-s MEEKC were more accurate. In addition, the err