纳米磁珠对单增李斯特菌低场磁共振检测的影响研究-微生物学专业论文.docxVIP

优秀毕业论文 精品参考文献资料 Abstract Abstract Shanghai Normal University Master of Philosophy method of上．monocytogenesin was successfully constructed by using different nano-materials．We found that dispersed nanoparticles in solution self-assemble on the surface of a bacterial target accompanied by the increase of the T2 value of water protons．We can determine the presence of L．monocytogeneson the basis of the change of T2 value(AT2)using NMR．Different nano—materials had a strong specificity for厶monocytogeneson．Through the comparative experiment，the biological function of Fe／Fe304 N-Ps and Fe304 NPs for magnetic resonance experiment for optimal stabilizer was low fat(2％)milk．The optimal concentration of Fe／Fe304 NPs Was 0．1 6 mg／mL and Fe304 NPs was 0．2mg／mL．The optimal incubation time of Fe／Fe304 NPs was 30min，while Fe304 NPs Was 40min，which was slightly shorter in the former．111e linear detection range of different nano-materials in the rapid detection of L．monocytogeneson Was 1-1 0’CFU／mL．The detection range was consistent．It showed that the sensitivity of the method Was not related to the nano-material itself． In conclusion，we have demonstrated the development of a highly specific， sensitive and fast method for the detection ofL．monocytogenesis using LF-NMR．We alSO explore the effect of LF-NMR of different nano-materials on the rapid deletion of L．monocytogenesin．111e experiments were used to increase the stability of this method，which had important significance for the rapid detection of foodbome pathogens in the future． Keyword：L．monocytogenes；low-field magnetic resonance technology；rapid detection：llano．materials；effect IV 万方数据 上海师范大学硕士学位论文 上海师范大学硕士学位论文 目录 目录 摘要 ．I Abstract ．．III 目录 ．V 第一章 绪论 ．1 1．1 单增李斯特菌检测方法的发展 ．．1 1．1．1单增李斯特菌在食品中的危害及现状 ．．1 1．1．2单增李斯特菌的检测方法 2 1．1．3各种检测方法的特点 ．7 1．2核磁共振技术的研究 ．8 1．2．1纳米技术的研究 ．．8 1．2．2磁性纳米粒子的合成和表面修饰 ．．9 1．2．3核磁共振技术在检测中的研究进展 1l 1．3 本论文的研究目的和研究内容 ．16 第二章 生物功能化磁性纳米材料的合