玻璃纳米孔洞电极单分子检测研究及碳纤维超微电极制备分析化学专业论文.docxVIP

AbstractAbstract Abstract Abstract This work reviewed the recent progress on single molecule detection by nanopores and the application of carbon fiber electrodes，and aimed at the preparation， characterization and application of new type of glass nanopore electrode and a glass encapsulated carbon fmer ultramicro electrodes．Scanning electron microscope and electrochemical methods were mainly used to characterize electrodes．The details were as follows： A new and simple way of preparing nanopore electrodes with glass capillaries for single molecule detection was developed．The tip of glass capillary Was melted over an alcohol burner and then polished to open on san@apers．The prepared nanopore electrodes were mechanically strong，easy to handle，and could generate current pulses with high signal-to-noise ratio．The model molecule 13-cyclodextrin(13-CD)was used tO characterize the basic function of the nanopore．When single 13-CD molecule passed through the nanopore，current pluses were generated with the pulse amplitude of 2．30 pA and the noise levellower then 0．5 pA．Besides，the maximum retention time of a single p-CD in the channel could be up tO 1 6 s，which shows the power to keep a single molecule in the nano·channel for further study．We also found that both positive and negative potential settings could generated single molecule signals in the experimental condition that the diffusion gradient was from outside to inside of the nanopore．Two typical amplitudes of the current pulses for single 13-CD molecule passing through the nanopore were observed and assigned to two orientations of the 13-CD molecules in the glass nanopore．Under the arrangement that the direction of electroosmosis flow was reversed with the diffusion gradient of B-CD，the ret。ention time cail be adjusted by changing the applied potential of the nanopore eleqtrode， which WaS significant in practical applications of the nanopore．In addition，the inner surface of the glass nanopore was treated by amino silane