放射性核素标记三肽ret和reg在肺癌分子显像中的实验研究影像医学与核医学专业论文.docxVIP

中文摘要 中文摘要 放射性核素标记三肽RET和REG在肺癌分子显像中的实验研究 比值(T／NT)为3．50±1．27。肝脏、脾脏有较多放射性摄取，且呈缓慢下降，肾脏、 心、肺放射性摄取随时间逐渐下降，其余组织放射性呈低水平。尾静脉注射99Tcm_RET 后4h荷瘤裸鼠显像示肿瘤显影清晰，此时体内分布研究示肿瘤％ID／g达4．96±1．05， 肝脏、脾脏有较多放射性摄取，而心脏和血液内放射性摄取较少。肿瘤与心脏和血液 的放射性计数比值(T／NT)最高可达5．7±O．21和12．4±0．11。 结论：99Tcm．RET和99Tcm_REG易于制备，99Tcm-RET对肺癌细胞H1299的亲和 力较99Tcm_REG高，具有较理想的动物体内动力学，对肿瘤的高亲和性，具有潜在的 新型靶向性显像药物，有可能成为一种亲肺癌肿瘤显像剂。 关键词：多肽，锝，肺癌，同位素标记，药代动力学 作 者：谢文晖 指导老师：吴翼伟 万方数据 放射性核素标记三肽RET和REG在肺癌分子显像中的实验研究 放射性核素标记三肽RET和REG在肺癌分子显像中的实验研究 英文摘要 Preparation of radionuclide labeled peptides RET and REG and the Experimental study on molecular imaging in nude mice bearing human lung cancer Abstract Objective To investigate the biodistribution characteristics and pharmacokinetics in nomal mice and uptake in human lung cancer cells of 99Tcm labeled arginine—glutamic—glycine(REG)and 99Tcm labeled arginine—glutamic—glutamic(RET)． Methods REG and RET was labeled directly with 99Tcm．The biodistribution of 99Tcm—REG and99TcmRET were studied at 2 min，5 min，1 0 min，30 min，1 h，2 h，4 h，8 h， and 24h，respectively，after intravenous injection in normal mice．At different time points， the mice were sacrificed and organs of interest were excised，weighted and counted by a gamma counter．The organ uptake was calculated aS a％ID／g．The scintigraphy of 99TcmREG was performed at 0．5，1，2，4．5，5，6h after intravenous injection．Results The radiochemical purity of 99TcmREG were more than 90％，and the specific binding efficiency of99Tcm．REG with A549 and H1299 cells Was(1．29-a：0．16)％and(2．32-士0．31)％． The％ID／g of tumor Was 1．954-0．73 at l h after injection．The tumor in nude mice could be best imaged at 2-6h after intravenous injection of 99Tcm-REG．In the first 24h after injection，the biodistribution in mice showed rapid blood excretion and most of the radioactivity Was observed in liver．The radiochemical purity of 99Tcm-RET were more than 90％，and the specific binding efficiency of 99Tcm．RET with H1 299 cells