大分子印迹交联聚乙烯基吡咯烷酮微球的制备与特性研究材料学专业论文.docxVIP

摘要以N．乙烯基吡咯烷酮(NVP)为单体，羟乙基纤维素(HEC)为分散剂， 摘要 以N．乙烯基吡咯烷酮(NVP)为单体，羟乙基纤维素(HEC)为分散剂， N，N．亚甲基双丙烯酰胺(bisAM)为交联剂，过氧化苯甲酰／二甲基苯胺 (BPO／DMA)为引发剂，牛血清蛋白(BSA)为模板，采用低温悬浮聚合方法， 制备了平均粒径为2009m的牛血清蛋白大分子印迹交联聚乙烯基吡咯烷酮微球 (BSA-IMs)。讨论了影响BSA．LMs粒径大小、分布和形态的各种因素：水油比、 分散剂的种类及加入量、搅拌速度、引发剂的用量及加入方式、反应温度等。 BSA．IMs的重结合行为测试结果表明，对牛血清蛋白有较好的特异选择性能，吸 附平衡时的印迹效率(IE)可达到3．25。反应温度、BSA中加入盐的浓度、BSA 溶液pH值、凝胶微球中模板的洗脱方式对BSA—IMs的吸附量和印迹效率都有不 同程度的影响。反应温度会影响微球的形态进而问接影响吸附性能，随着反应温 度的升高印迹效率稍有下降；BSA溶液中盐浓度达到某一值时，BSA．1Ms的吸附 量出现了极大值，而印迹效率呈现出逐渐下降的趋势；BSA溶液的pH值越低， BSA—IMs的吸附量也越大，但印迹效率逐渐降低；模板的洗脱方式对BSA．IMs的 吸附量也有很大影响，用含1．0％SDS的10％乙酸(VⅣ)水溶液洗脱液对BSA．IMs 进行洗脱，BSA．IMs体现出了较高的吸附量和印迹效率。 关键词：大分子印迹；分子印迹交联聚乙烯基吡咯烷酮微球；低温悬浮聚合；牛 血清蛋白；重结合；平衡吸附量；印迹效率 ABSTRACTUsing ABSTRACT Using N—vinylpyrrolidone(NVP)as the functional monomer,N，N-methylene． bisacrylamide(BisAM)as crosslinker,benzoyl peroxide／amine(BPO／DMA)as the redox initiator system and bovine serum albumin(BSA)as template，via suspension polymerization，we prepared protein-imprinted crosslinked polyvinyl．pyrrolidone (PVP)hydrogel microspheres and with the diameter of the microspheres being 200 pm．The effects of the ratio between the continuous phase and the disperse phase，the stiring speed，the kind and quantity of the stabilizers，the polymerization temperature on the size，size distribution and morphology of the crosslinked PVP microspheres in the process of polymerization were investigated． The adsorption and recognition properties of the microspheres were studied，as well as the imprinting mechanism was discussed．The selectivity test of imprinted microspheres showed that the imprinted microspheres exhibited good recognition for BSA．The adsorption capacity of BSA．imprinted microspheres was 3．25 times more than that of non—imprinted microspheres．It was considered that the f．o加ation of multiple hydrogen bonds and complementary shape between the imprinting cavities and the template proteins were the two factors that led to the imprinting effect． The pH of BSA solution，the ionic strength，the removing metho