当归四逆汤加减方对大鼠糖尿病神经病变的作用及机制研究内科学内分泌与代谢性疾病专业论文.docxVIP

摘要关键词：当归四逆汤加减方；糖尿病神经病变；氧化硝化应激 摘要 关键词：当归四逆汤加减方；糖尿病神经病变；氧化硝化应激 II 万方数据 AbstractAbstract Abstract Abstract Objeetive---Diabetic neuropathy(DN)is one of common complications of diabetes． Our previous studies found that Danggui Sini Decoction(DSD)carl relieve the pain caused by diabetic neuropathy．To observe the effects of Danggui Sini Decoction (DSD)on hind legs of footpad intraepidermal nerve fibeL spinal cord and sciatic nerve nitric oxide(NO)，inducible nitric oxide synthase(iNOS)，Antioxidant enzymes，apoptosis-related proteins and nitrotyrosine(NT)expression in diabetic SD rats． Methods--Type I diabetic rats were induced by Streptozotocin(STZ)．Rats were randomized to 3 groups：control group，diabetic group，DSD group．Rats were gavaged for 1 2 weeks．We detcet the weight，blood glucose，the tail length，the body length and the tail root circumference．Using immunohistochemical methods to detect the expression of iNOS，n．NOS，S-1 00，NT of spinal cord and sciatic nerve and hind legs footpad intraepidermal nerve fiber by PGP9．5 antibody．Using Real．time Quantitative PCR Detecting System to detect the mRNA expression of iNOS，Antioxidant enzymes(Mn—SOD，Cu-Zn-SOD)，apoptosis-related proteins (Bcl-2，Bax)in spinal cord．Using Nitric oxide detection kit detected toted NO． Results—Compared with the control group，The expression of iNOS，NT in sciatic nerve were increased(p0．05)，as well as the mRNA expression of iNOS． Mn·SOD，Cu-Zn_SOD in spinal cord were increased(p0．05)，the mRNA expression of Bcl-2／Bax n spinal cord were decreased(p0．05)，and the hind legs footpad intraepidermal nerve fiber area in diabetic rats Was decreased(p0．05)；Compared with diabetic group，the expression of iNOS and NT in sciatic nerve of DSD rats were lowered(p0．05)，and the mRNA expression of iNOS，Mn．SOD。Cu．Zn．SOD in spinal cord of DSD group were increased(p0．05)，the hind 1egs footpad intraepiderrnal nerve fiber area in DSD rats was increased(p0．05)．Compared witll the diabetic group，There was no signific