茶多酚高通量检测技术研究进展.PDF

中国农学通报 2019,35(3): 146-153 Chinese Agricultural Science Bulletin 茶多酚高通量检测技术研究进展 林清霞，项丽慧，王丽丽，杨军国，陈 林 （福建省农业科学院茶叶研究所，福建福安355015） 摘 要：茶多酚为茶叶主要活性成分，实现其组分的高通量快速检测，对茶叶加工品质控制和产品质量 评价具有重要指导意义。对茶多酚高通量快速检测技术及其相关应用进行了归纳和总结，详细阐述了 高效液相色谱法及其影响因素（色谱柱、检测器、样品前处理、流动相、洗脱方式），亦对毛细管电泳法、近 红外光谱法、核磁共振法等检测方法进行简述，并分析各方法的优势及存在问题。研究认为，茶多酚高 通量检测方法仍普遍存在程序繁琐、设备成本高昂、有机溶剂耗量大、检测灵敏度低等问题，在此基础提 出液质联用技术的产业化发展，同时经济环保的实时在线监测以及复杂基质中茶多酚组分的快速定性 定量是茶多酚高通量检测技术未来重点发展方向。 关键词：茶多酚；高通量；检测技术；综述 中图分类号：TS272.2 文献标志码：A 论文编号：cas HighThroughputTestofTeaPolyphenols:AReview LinQingxia,XiangLihui,WangLili,YangJunguo,ChenLin (TeaResearchInstitute,FujianAcademyofAgriculturalScience,Fu’anFujian355015) Abstract:Teapolyphenolsarethemainactiveingredientsoftea.Thehighthroughputandrapid test oftea polyphenolshasguidingsignificanceforteaprocessingqualitycontrolandteaqualityevaluation.So,thestudy reviewed the high throughput test technique and its application to tea polyphenols, described the high performanceliquidchromatographyanditsinfluencingfactors(suchascolumn,detector,samplepretreatment, mobilephaseandelutionmethod),and briefed otherdetectionmethodsincludingcapillaryelectrophoresis, nearinfraredspectroscopyandnuclearmagneticresonance.Additionally,thestudyanalyzedtheadvantages and existing problemsofthesemethods.Thestudyindicatedthatthehighthroughputtestofteapolyphenols still had some problems such as complex procedures, high cost, high organic solvent consumption, low sensitivityandsoon.Basedonthe analysis,thestudyproposestheindustrialization development ofliquid- masstechnology,and pointsoutthateconomical andenvironment-friendly real-timeonlinemonitoring,and rapidqualitativeandquantitativeanalysisofteapolyphenolsincomplexsubstratesare