不同草莓CHLH基因序列及其内含子信息差异分析.PDF

中国农学通报 2012,28(01):228-232 Chinese Agricultural Science Bulletin 不同草莓CHLH 基因序列及其内含子信息差异分析 张 希，葛安静，董清华 （北京农学院植物科学技术学院，北京102206） 摘 要：Mg2+螯合酶H 亚基(CHLH)是一种ABA 特异性结合蛋白。为进一步研究ABA 结合蛋白在抗逆性 方面的作用，利用GenBank 数据库中草莓Mg2 + 螯合酶H 亚基基因CHLH 的cDNA 序列（登录号为 HQ829473）信息，采用同源克隆法从3 种不同草莓品种栽培八倍体‘红颜’草莓、野生二倍体森林草莓和 野生二倍体五叶草莓叶片中克隆获得了CHLH 基因DNA 序列。基因结构分析表明，CHLH 包含有个5 个外显子和4 个内含子，4 个内含子的变化范围为109~486 bp ，其中五叶草莓的第2 个和第4 个内含子序 列上不同于另外2 种。内含子参与基因表达调节，说明五叶草莓可能在内含子调控作用上有不同之处。 关键词：草莓；ABA 受体；CHLH 基因；CHLH 内含子 中图分类号：S668.4 文献标志码：A 论文编号：2011-2090 CHLH Gene Sequence and Variance Analysis of Intron Information in Different Fragria × ananassa Zhang Xi, Ge Anjing, Dong Qinghua (Plant Science and Technology College, Beijing University of Agriculture, Beijing 102206) Abstract: Mg2 + chelatase H subunit (CHLH) is an ABA specific binding protein. To study the role in the resistance of abscisic acid binding protein further, use the cDNA sequence information (accession No. HQ829473) of Mg2 + chelatase H subunit CHLH of Fragria ×ananassa in the GenBank databas, and get DNA sequence of CHLH with homology-based cloning method from three different species Fragria×ananassa leaves. The analysis of gene structure showed that, CHLH contained five exons and four introns, four introns vary from 109-486 bp; and Fragaria pentaphylla Lozinsk was different from others in the first and fourth introns. Intron involved in regulation of gene expression, it said that Fragaria pentaphylla Lozinsk may have different function in regulation of intron. Key words: Fragria×ananassa; abscisic acid binding protein; CHLH; CHLH intron [2-3] 0 引言